Detail of 20130412_1715_58_Graft4th_x8000

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Project
Title
Transmission electron microscopy (TEM) image of retinal tissue from Nrl-GFP mouse induced pluripotent stem cells (miPSC) at differentiation day (DD) 17
Description
NA
Release, Updated
2017-10-03,
2018-11-15
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
4.0 MB

Organism
M. musculus ( NCBI:txid10090 )
Strain(s)
iPS
Cell Line
-
Gene symbols
Nrl
Protein tags
GFP

Datatype
tissue structure
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
cell ( GO:0005623 )
Biological Imaging Method
XYZ Scale
XY: 0.00283 micrometer/pixel, Z: NA
T scale
-

Image Acquisition
Experiment type
Other
Microscope type
Other
Acquisition mode
Other
Contrast method
Other
Microscope model
JEOL JEM 1010 or JEM 1400
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Assawachananont et al. (2014) Stem Cell Reports, 2(5): 662-674.
Related paper(s)

Juthaporn Assawachananont, Michiko Mandai, Satoshi Okamoto, Chikako Yamada, Mototsugu Eiraku, Shigenobu Yonemura, Yoshiki Sasai, Masayo Takahashi (2014) Transplantation of embryonic and induced pluripotent stem cell-derived 3D retinal sheets into retinal degenerative mice., Stem cell reports, Volume 2, Number 5, pp. 662-74

Published in 2014 May 6 (Electronic publication in April 24, 2014, midnight )

(Abstract) In this article, we show that mouse embryonic stem cell- or induced pluripotent stem cell-derived 3D retinal tissue developed a structured outer nuclear layer (ONL) with complete inner and outer segments even in an advanced retinal degeneration model (rd1) that lacked ONL. We also observed host-graft synaptic connections by immunohistochemistry. This study provides a "proof of concept" for retinal sheet transplantation therapy for advanced retinal degenerative diseases.
(MeSH Terms)

Contact
Michiko Mandai , RIKEN , Center for Developmental Biology , Laboratory for Retinal Regeneration
Contributors
Juthaporn Assawachananont, Michiko Mandai, Satoshi Okamoto, Chikako Yamada, Mototsugu Eiraku, Shigenobu Yonemura, Yoshiki Sasai, Masayo Takahashi

OMERO Dataset
OMERO Project
Source