Detail of Fig7C_HEK293T_PA-Gal4ccH
Project
Title
Time-lapse images of PA-Gal4ccH expression by patterned light illumination of HEK293T cells.
Description
Time-lapse images of PA-Gal4ccH expression by patterned light illumination of HEK293T cells.
Release, Updated
2022-11-23
License
CC BY-NC
Kind
Image data
File Formats
uncompressed TIFF
Data size
262.0 MB
Reporter
PA-Gal4cc
Datatype
-
Molecular Function (MF)
translation activator activity
(
GO:0008494
)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
time lapse microscopy
(
Fbbi:00000249
)
X scale
0.75 micrometer/pixel
Y scale
0.75 micrometer/pixel
Z scale
-
T scale
5 minutes per time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Yamada M, et. al. (2020) iScience, 2020 Aug 27;23(9):101506.
Related paper(s)
Mayumi Yamada, Shinji C Nagasaki, Yusuke Suzuki, Yukinori Hirano, Itaru Imayoshi (2020) Optimization of Light-Inducible Gal4/UAS Gene Expression System in Mammalian Cells., iScience, Volume 23, Number 9, pp. 101506
Published in 2020 Aug 27 (Electronic publication in Aug. 27, 2020, midnight )
- doi: 10.1016/j.isci.2020.101506
-
pmid: 32919371
(Abstract) Light-inducible gene expression systems represent powerful methods for studying the functional roles of dynamic gene expression. Here, we developed an optimized light-inducible Gal4/UAS gene expression system for mammalian cells. We designed photoactivatable (PA)-Gal4 transcriptional activators based on the concept of split transcription factors, in which light-dependent interactions between Cry2-CIB1 PA-protein interaction modules can reconstitute a split Gal4 DNA-binding domain and p65 transcription activation domain. We developed a set of PA-Gal4 transcriptional activators (PA-Gal4cc), which differ in terms of induced gene expression levels following pulsed or prolonged light exposure, and which have different activation/deactivation kinetics. These systems offer optogenetic tools for the precise manipulation of gene expression at fine spatiotemporal resolution in mammalian cells.
Contact
Itaru Imayoshi
, kyoto University
, Graduate School of Biostudies
, Research Center for Dynamic Living Systems
Contributors
OMERO Dataset
OMERO Project
Source