Detail of Figure4B_Brafv600E_mice_tumor
Project
Title
Bioluminescence imaging of B6 albino mice subcutaneously implanted with parental, Ifngr1–/–, Gnaq–/–, or Ifngr1–/–/ Gnaq–/– BrafV600E melanoma cells expressing ISP-GAS or ISP-ISRE from Day 3 to Day 7.
Description
Bioluminescence imaging of B6 albino mice subcutaneously implanted with parental, Ifngr1–/–, Gnaq–/–, or Ifngr1–/–/ Gnaq–/– BrafV600E melanoma cells expressing ISP-GAS or ISP-ISRE from Day 3 to Day 7.
Release, Updated
2022-11-23
License
CC BY
Kind
Image data
File Formats
.tif
Data size
132.7 MB
Gene symbols
Ifngr1, Gnaq
Datatype
-
Molecular Function (MF)
IFN binding
(
GO:0019961
)
interferon-gamma binding
(
GO:0019964
)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
X scale
500 micrometer/pixel
Y scale
500 micrometer/pixel
Z scale
-
T scale
30 sec interval in each file. 1 day per time interval.
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Tanaka T, et. al. (2021) Cell Struct Funct, Dec 22;46(2):103-111.
Related paper(s)
Taisei Tanaka, Yoshinobu Konishi, Hiroshi Ichise, Shinya Tsukiji, Michiyuki Matsuda, Kenta Terai (2021) A Dual Promoter System to Monitor IFN-gamma Signaling in vivo at Single-cell Resolution., Cell structure and function, Volume 46, Number 2, pp. 103-111
Published in 2021 Dec 22 (Electronic publication in Nov. 6, 2021, midnight )
- doi: 10.1247/csf.21052
-
pmid: 34744115
(Abstract) IFN-gamma secreted from immune cells exerts pleiotropic effects on tumor cells, including induction of immune checkpoint and antigen presentation, growth inhibition, and apoptosis induction. We combined a dual promoter system with an IFN-gamma signaling responsive promoter to generate a reporter named the interferon sensing probe (ISP), which quantitates the response to IFN-gamma by means of fluorescence and bioluminescence. The integration site effect of the transgene is compensated for by the PGK promoter-driven expression of a fluorescent protein. Among five potential IFN-gamma-responsive elements, we found that the interferon gamma-activated sequence (GAS) exhibited the best performance. When ISP-GAS was introduced into four cell lines and subjected to IFN-gamma stimulation, dose-dependency was observed with an EC50 ranging from 0.2 to 0.9 ng/mL, indicating that ISP-GAS can be generally used as a sensitive biosensor of IFN-gamma response. In a syngeneic transplantation model, the ISP-GAS-expressing cancer cells exhibited bioluminescence and fluorescence signals in an IFN-gamma receptor-dependent manner. Thus, ISP-GAS could be used to quantitatively monitor the IFN-gamma response both in vitro and in vivo.Key words: in vivo imaging, tumor microenvironment, interferon-gamma, dual promoter system.(MeSH Terms)
Contact
Kenta Terai
, kyoto University
, Graduate School of Medicine
, Department of Pathology and Biology of Diseases
Contributors
Kenta Terai, Taisei Tanaka
OMERO Dataset
OMERO Project
Source