Detail of SuppFig7_gecko_embryo_movie1

(Too many images for preview; see images in SSBD:OMERO Dataset)


Project
Title
Live-imaging of the absence of motile cilia in the middle of gecko embryos at stage 9.
Description
Live-imaging of the absence of motile cilia in the middle of gecko embryos at stage 9.
Release, Updated
2022-03-31
License
CC BY
Kind
Image data
File Formats
.avi
Data size
1.8 GB

Organism
Gekko ( NCBI:txid8565 )
Strain(s)
-
Cell Line
-

Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
left/right axis specification ( GO:0070986 )
Cellular Component (CC)
motile cilium ( GO:0031514 ) non-motile cilium ( GO:0097730 )
Biological Imaging Method
differential interference contrast microscopy ( Fbbi:00000245 )
X scale
0.12077 micrometer/pixel
Y scale
0.12077 micrometer/pixel
Z scale
-
T scale
150 frames per second

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Kajikawa E, et. al. (2020) Nat Ecol Evol., 4(2):261-269.
Related paper(s)

Eriko Kajikawa, Uzuki Horo, Takahiro Ide, Katsutoshi Mizuno, Katsura Minegishi, Yuichiro Hara, Yayoi Ikawa, Hiromi Nishimura, Masanori Uchikawa, Hiroshi Kiyonari, Shigehiro Kuraku, Hiroshi Hamada (2020) Nodal paralogues underlie distinct mechanisms for visceral left-right asymmetry in reptiles and mammals., Nature ecology & evolution, Volume 4, Number 2, pp. 261-269

Published in 2020 Feb (Electronic publication in Jan. 6, 2020, midnight )

(Abstract) Unidirectional fluid flow generated by motile cilia at the left-right organizer (LRO) breaks left-right (L-R) symmetry during early embryogenesis in mouse, frog and zebrafish. The chick embryo, however, does not require motile cilia for L-R symmetry breaking. The diversity of mechanisms for L-R symmetry breaking among vertebrates and the trigger for such symmetry breaking in non-mammalian amniotes have remained unknown. Here we examined how L-R asymmetry is established in two reptiles, Madagascar ground gecko and Chinese softshell turtle. Both of these reptiles appear to lack motile cilia at the LRO. The expression of the Nodal gene at the LRO in the reptilian embryos was found to be asymmetric, in contrast to that in vertebrates such as mouse that are dependent on cilia for L-R patterning. Two paralogues of the Nodal gene derived from an ancient gene duplication are retained and expressed differentially in cilia-dependent and cilia-independent vertebrates. The expression of these two Nodal paralogues is similarly controlled in the lateral plate mesoderm but regulated differently at the LRO. Our in-depth analysis of reptilian embryos thus suggests that mammals and non-mammalian amniotes deploy distinct strategies dependent on different Nodal paralogues for rendering Nodal activity asymmetric at the LRO.
(MeSH Terms)

Contact
Shigehiro Kuraku, Hiroshi Hamada , RIKEN, RIKEN , Center for Biosystems Dynamics Research, Center for Biosystems Dynamics Research , Laboratory for Phyloinformatics, Laboratory for Organismal Patterning
Contributors

OMERO Dataset
OMERO Project
Source