Detail of FigS6I-K_hippocampal_dentate_7day
Project
Title
Immunostaining images of cells in the adult mouse hippocampal dentate gyrus infected with hGAVPO-mCherry virus and UAS-Ascl1-E47-Venus virus after 7-day light stimulation with 2.5 h periodicity.
Description
Immunostaining images of cells in the adult mouse hippocampal dentate gyrus infected with hGAVPO-mCherry virus and UAS-Ascl1-E47-Venus virus after 7-day light stimulation with 2.5 h periodicity.
Release, Updated
2022-03-31
License
CC BY
Kind
Image data
File Formats
.lsm
Data size
4.1 MB
Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
gene expression
(
GO:0010467
)
regulation of gene expression
(
GO:0010468
)
Cellular Component (CC)
-
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
X scale
0.8302658 micrometer/pixel
Y scale
0.8302658 micrometer/pixel
Z scale
2 micrometer/slice
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Sueda R, et. al. (2019) Genes Dev., 33(9-10):511-523.
Related paper(s)
Risa Sueda, Itaru Imayoshi, Yukiko Harima, Ryoichiro Kageyama (2019) High Hes1 expression and resultant Ascl1 suppression regulate quiescent vs. active neural stem cells in the adult mouse brain., Genes & development, Volume 33, Number 9-10, pp. 511-523
Published in 2019 May 1 (Electronic publication in March 12, 2019, midnight )
- doi: 10.1101/gad.323196.118
-
pmid: 30862661
(Abstract) Somatic stem/progenitor cells are active in embryonic tissues but quiescent in many adult tissues. The detailed mechanisms that regulate active versus quiescent stem cell states are largely unknown. In active neural stem cells, Hes1 expression oscillates and drives cyclic expression of the proneural gene Ascl1, which activates cell proliferation. Here, we found that in quiescent neural stem cells in the adult mouse brain, Hes1 levels are oscillatory, although the peaks and troughs are higher than those in active neural stem cells, causing Ascl1 expression to be continuously suppressed. Inactivation of Hes1 and its related genes up-regulates Ascl1 expression and increases neurogenesis. This causes rapid depletion of neural stem cells and premature termination of neurogenesis. Conversely, sustained Hes1 expression represses Ascl1, inhibits neurogenesis, and maintains quiescent neural stem cells. In contrast, induction of Ascl1 oscillations activates neural stem cells and increases neurogenesis in the adult mouse brain. Thus, Ascl1 oscillations, which normally depend on Hes1 oscillations, regulate the active state, while high Hes1 expression and resultant Ascl1 suppression promote quiescence in neural stem cells.(MeSH Terms)
- Animals
- Basic Helix-Loop-Helix Transcription Factors/genetics[major]
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- Brain/cytology[major]
- Gene Expression Profiling
- Gene Expression Regulation/genetics[major]
- Gene Silencing
- Mice
- Neural Stem Cells/cytology[major]
- Neural Stem Cells/metabolism[major]
- Neurogenesis/genetics[major]
- Optogenetics
- Promoter Regions, Genetic
- Transcription Factor HES-1/genetics[major]
- Transcription Factor HES-1/metabolism
Contact
Ryoichiro Kageyama, Itaru Imayoshi
, Kyoto University, Kyoto University
, Institute for Frontier Life and Medical Sciences, Institute for Frontier Life and Medical Sciences
Contributors
OMERO Dataset
OMERO Project
Source