Detail of Fig3A_MNI-Glu-Minus
Project
Title
Two-photon RICS images of cultured hippocampal neurons expressing EGFP5 in mouse spine structural without glutamate induction.
Description
Two-photon RICS images of cultured hippocampal neurons expressing EGFP5 in mouse spine structural without glutamate induction.
Release, Updated
2022-03-31
License
CC BY
Kind
Image data
File Formats
.tif
Data size
193.1 KB
Protein tags
EGFP5
Datatype
-
Molecular Function (MF)
signaling molecule
(
GO:0048018
)
Biological Process (BP)
actin polymerization
(
GO:0030041
)
stimulation of synapse structural plasticity
(
GO:0051835
)
Cellular Component (CC)
dendritic spine
(
GO:0043197
)
Biological Imaging Method
two-photon laser scanning microscopy
(
Fbbi:00000254
)
X scale
0.206 micrometer/pixel
Y scale
0.206 micrometer/pixel
Z scale
-
T scale
-20, -10, -1, 1, 15, 30 minutes
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Obashi K, et. al. (2019) Cell Rep., 27(5):1503-1515.
Related paper(s)
Kazuki Obashi, Atsushi Matsuda, Yasuhiro Inoue, Shigeo Okabe (2019) Precise Temporal Regulation of Molecular Diffusion within Dendritic Spines by Actin Polymers during Structural Plasticity., Cell reports, Volume 27, Number 5, pp. 1503-1515.e8
Published in 2019 Apr 30
- doi: 10.1016/j.celrep.2019.04.006
-
pmid: 31042476
(Abstract) The biochemical transduction of excitatory synaptic signals occurs in the cytoplasm within dendritic spines. The associated reaction kinetics are shaped by the mobility of the signaling molecules; however, accurate monitoring of diffusional events within the femtoliter-sized spine structures has not yet been demonstrated. Here, we applied two-photon fluorescence correlation spectroscopy and raster image correlation spectroscopy to monitor protein dynamics within spines, revealing that F-actin restricts the mobility of proteins with a molecular mass of >100 kDa. This restriction is transiently removed during actin remodeling at the initial phase of spine structural plasticity. Photobleaching experiments combined with super-resolution imaging indicate that this increase in mobility facilitates molecular interactions, which may modulate the functions of key postsynaptic signaling molecules, such as Tiam1 and CaMKII. Thus, actin polymers in dendritic spines act as precise temporal regulators of molecular diffusion and modulate signal transduction during synaptic plasticity.(MeSH Terms)
- Actin Cytoskeleton/metabolism[major]
- Actins/metabolism[major]
- Animals
- Biological Transport
- Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism
- Cells, Cultured
- Dendritic Spines/metabolism[major]
- Dendritic Spines/ultrastructure
- Diffusion
- Female
- Male
- Mice
- Mice, Inbred ICR
- Neuronal Plasticity[major]
- Signal Transduction
- T-Lymphoma Invasion and Metastasis-inducing Protein 1/metabolism
Contact
Shigeo Okabe
, the University of Tokyo, Tokyo
, Department of Cellular Neurobiology, Graduate School of Medicine
Contributors
Kazuki Obashi
OMERO Dataset
OMERO Project