Detail of FigureS3BC_BC_KT-MT_20160208_half
Project
Title
Immunostaining images of kinetochores (red) and microtubules (green) of halved cytoplasmic mouse oocytes during meiosis
Description
Immunostaining images of kinetochores (red) and microtubules (green) of halved cytoplasmic mouse oocytes during meiosis
Release, Updated
2021-11-30
License
CC BY
Kind
Image data
File Formats
Data size
800.0 MB
Datatype
-
Molecular Function (MF)
-
Biological Process (BP)
nuclear envelope breakdown
(
GO:0051081
)
mitotic cell cycle
(
GO:0007067
)
cytoplasm organization
(
GO:0007028
)
Cellular Component (CC)
kinetochore microtubule
(
GO:0005828
)
chromosome
(
GO:0005694
)
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
X scale
0.0691888 micrometer/pixel
Y scale
0.0691888 micrometer/pixel
Z scale
0.3791020 micrometer/slice
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Kyogoku H, et. al. (2017) Dev. Cell, 41(3): 287-298.
Related paper(s)
Hirohisa Kyogoku, Tomoya S Kitajima (2017) Large Cytoplasm Is Linked to the Error-Prone Nature of Oocytes., Developmental cell, Volume 41, Number 3, pp. 287-298.e4
Published in 2017 May 8
- doi: 10.1016/j.devcel.2017.04.009
-
pmid: 28486131
(Abstract) Chromosome segregation during meiosis in oocytes is error prone. The uniquely large cytoplasmic size of oocytes, which provides support for embryogenesis after fertilization, might be a predisposing factor for meiotic errors. However, this hypothesis remains unproven. Here, we show that cytoplasmic size affects the functionality of the acentrosomal spindle. Artificially decreasing the cytoplasmic size in mouse oocytes allows the acentrosomal spindle poles to have a better-focused distribution of microtubule-organizing centers and to biorient chromosomes more efficiently, whereas enlargement of the cytoplasmic size has the opposite effects. Moreover, we found that the cytoplasmic size-dependent dilution of nuclear factors, including anaphase inhibitors that are preformed at the nuclear membrane, limits the spindle's capacity to prevent anaphase entry with misaligned chromosomes. The present study defines a large cytoplasmic volume as a cell-intrinsic feature linked to the error-prone nature of oocytes. This may represent a trade-off between meiotic fidelity and post-fertilization developmental competence.(MeSH Terms)
Contact
Tomoya S. Kitajima
, RIKEN
, Center for Biosystems Dynamics Research
, Laboratory for Chromosome Segregation
Contributors
NA
OMERO Dataset
OMERO Project
Source