SSBD:database

Thumbnail for FigureS5E_150118_mNeonGreen-Nsl1_B56e-mNeonGreen-Nsl1_H2B-mCherry

Detail of FigureS5E_150118_mNeonGreen-Nsl1_B56e-mNeonGreen-Nsl1_H2B-mCherry

Project

SSBD:Repository

Title

Timelapse confocal images of oocytes expressing mNeonGreen-Nsl1, PP2A-B56ε-mNeonGreen, or PP2A-B56ε-mNeonGreen-Nsl1 (green) and H2B-mCherry (red) 4 h after NEBD

Description

Timelapse confocal images of oocytes expressing mNeonGreen-Nsl1, PP2A-B56ε-mNeonGreen, or PP2A-B56ε-mNeonGreen-Nsl1 (green) and H2B-mCherry (red) 4 h after NEBD

Release, Updated

2021-09-30

License

CC BY

Kind

Image data

File Formats

Data size

31.0 GB

Organism

Mus musculus ( NCBI:txid10090 )

Strain(s)

Cell Line

Protein names

PP2A, H2B

Protein tags

mNeonGreen, mCherry

Datatype

Molecular Function (MF)

Biological Process (BP)

mitotic cell cycle ( GO:0007067 )

Cellular Component (CC)

kinetochore microtubule ( GO:0005828 ) , chromosome ( GO:0005694 )

Biological Imaging Method

time lapse microscopy ( Fbbi:00000249 )

X scale

0.0593047 micrometer/pixel

Y scale

0.0593047 micrometer/pixel

Z scale

1.5 micrometer/slice

T scale

5 minutes for each time interval

Image Acquisition

Experiment type

Microscope type

Acquisition mode

Contrast method

Microscope model

Detector model

Objective model

Filter set

Summary of Methods

See details in Yoshida S, et. al. (2015) Dev Cell., 33(5):589-602.