SSBD:database

Thumbnail for Figure5A_140416_KNL1pS24_ACA_BubR1-3D

Detail of Figure5A_140416_KNL1pS24_ACA_BubR1-3D

Project

SSBD:Repository

Title

Timelapse confocal images of oocytes expressing mEGFP-BubR1-3D (phosphomimetic aspartic acid mutants) at 4 hours after NEBD were stained for pS24-Knl1 (green), KTs (red), chromosomes (blue)

Description

Timelapse confocal images of oocytes expressing mEGFP-BubR1-3D (phosphomimetic aspartic acid mutants) at 4 hours after NEBD were stained for pS24-Knl1 (green), KTs (red), chromosomes (blue)

Release, Updated

2021-09-30

License

CC BY

Kind

Image data

File Formats

Data size

197.5 MB

Organism

Mus musculus ( NCBI:txid10090 )

Strain(s)

Cell Line

Gene symbols

BubR1, Knl1

Protein tags

mEGFP

Datatype

Molecular Function (MF)

Biological Process (BP)

mitotic cell cycle ( GO:0007067 )

Cellular Component (CC)

kinetochore microtubule ( GO:0005828 ) , chromosome ( GO:0005694 )

Biological Imaging Method

time lapse microscopy ( Fbbi:00000249 )

X scale

0.0593047 micrometer/pixel

Y scale

0.0593047 micrometer/pixel

Z scale

1 micrometer/slice

T scale

Image Acquisition

Experiment type

Microscope type

Acquisition mode

Contrast method

Microscope model

Detector model

Objective model

Filter set

Summary of Methods

See details in Yoshida S, et. al. (2015) Dev Cell., 33(5):589-602.