Detail of Ddi_cAR1_JT

(Too many images for preview; see images in SSBD:OMERO Dataset)


Project
Title
Image of single molecule dynamics in D. discoideum
Description
NA
Release, Updated
2015-10-03,
2018-11-15
License
CC BY-NC-SA
Kind
Image data based on Experiment related Quantitative data ; Ddi_cAR1_JT
File Formats
Data size
450.0 MB

Organism
D. discoideum ( NCBI:txid44689 )
Strain(s)
-
Cell Line
-

Datatype
single molecule dynamics
Molecular Function (MF)
Biological Process (BP)
cellular protein localization ( GO:0034613 )
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 0.0445 micrometer/pixel, Z: 0 micrometer/slice
T scale
0.033 second for each time interval

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Komatsuzaki et al. (2015) Small, 11(12):1396-1401
Related paper(s)

Akihito Komatsuzaki, Tatsuya Ohyanagi, Yoshikazu Tsukasaki, Yukihiro Miyanaga, Masahiro Ueda, Takashi Jin (2015) Compact halo-ligand-conjugated quantum dots for multicolored single-molecule imaging of overcrowding GPCR proteins on cell membranes., Small (Weinheim an der Bergstrasse, Germany), Volume 11, Number 12, pp. 1396-401

Published in 2015 Mar 25 (Electronic publication in Dec. 12, 2014, midnight )

(Abstract) To detect single molecules within the optical diffraction limit (< ca. 200 nm), a multicolored imaging technique is developed using Halo-ligand conjugated quantum dots (Halo-QDs; <6 nm in diameter). Using three types of Halo-QDs, multicolored single-molecule fluorescence imaging of GPCR proteins in Dictyostelium cells is achieved.
(MeSH Terms)

Contact
Takashi Jin , RIKEN , Quantitative Biology Center , Laboratory for Nano-Bio Probes
Contributors
Akihiro Komatsuzaki, Tatsuya Ohyanagi, Yoshikazu Tsukasaki, Yukihiro Miyanaga, Masahiro Ueda, Takashi Jin

OMERO Dataset
OMERO Project
Source