Detail of Ddi_cAR1_JT
Project
Title
Image of single molecule dynamics in D. discoideum
Description
NA
Release, Updated
2015-10-03,
2018-11-15
2018-11-15
License
CC BY-NC-SA
Kind
Image data
based on Experiment
related Quantitative data ;
Ddi_cAR1_JT
File Formats
Data size
450.0 MB
Datatype
single molecule dynamics
Molecular Function (MF)
Biological Process (BP)
cellular protein localization
(
GO:0034613
)
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 0.0445 micrometer/pixel, Z: 0 micrometer/slice
T scale
0.033 second for each time interval
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Komatsuzaki et al. (2015) Small, 11(12):1396-1401
Related paper(s)
Akihito Komatsuzaki, Tatsuya Ohyanagi, Yoshikazu Tsukasaki, Yukihiro Miyanaga, Masahiro Ueda, Takashi Jin (2015) Compact halo-ligand-conjugated quantum dots for multicolored single-molecule imaging of overcrowding GPCR proteins on cell membranes., Small (Weinheim an der Bergstrasse, Germany), Volume 11, Number 12, pp. 1396-401
Published in 2015 Mar 25 (Electronic publication in Dec. 12, 2014, midnight )
- doi: 10.1002/smll.201402508
-
pmid: 25504902
(Abstract) To detect single molecules within the optical diffraction limit (< ca. 200 nm), a multicolored imaging technique is developed using Halo-ligand conjugated quantum dots (Halo-QDs; <6 nm in diameter). Using three types of Halo-QDs, multicolored single-molecule fluorescence imaging of GPCR proteins in Dictyostelium cells is achieved.(MeSH Terms)
- Cell Membrane/metabolism[major]
- Cell Membrane/ultrastructure
- Fluorescent Dyes/chemistry
- Halogens/chemistry[major]
- Ligands
- Microscopy, Fluorescence, Multiphoton/methods[major]
- Molecular Imaging/methods[major]
- Nanoconjugates/chemistry
- Nanoconjugates/ultrastructure
- Quantum Dots[major]
- Receptors, G-Protein-Coupled/metabolism[major]
- Reproducibility of Results
- Sensitivity and Specificity
Contact
Takashi Jin
, RIKEN
, Quantitative Biology Center
, Laboratory for Nano-Bio Probes
Contributors
Akihiro Komatsuzaki, Tatsuya Ohyanagi, Yoshikazu Tsukasaki, Yukihiro Miyanaga, Masahiro Ueda, Takashi Jin
OMERO Dataset
OMERO Project
Source