Detail of Supp_movie_3
Project
Title
Time-lapse images of intracellular Ca2+ release in mouse ESCs-induced salivary gland primordium (iSG) induced by 100 μM Charbacol after iSGs were pretreated with atropine
Description
NA
Release, Updated
2020-12-23
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
287.4 MB
Datatype
organogenesis
Molecular Function (MF)
Biological Process (BP)
salivary gland development
(
GO:0007431
)
submandibular salivary gland formation
(
GO:0060661
)
Cellular Component (CC)
nucleus
(
GO:0005634
)
Biological Imaging Method
XYZ Scale
XY: 0.6214806 micrometer/pixel, Z: 1 micrometer/slice
T scale
1.05 second for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
FluorescenceMicroscope
Acquisition mode
Other
Contrast method
Fluorescence
Microscope model
Keyence BZ-9000
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Tanaka et al. (2018) Nat. Commun., 9(1): 4216.
Related paper(s)
Junichi Tanaka, Miho Ogawa, Hironori Hojo, Yusuke Kawashima, Yo Mabuchi, Kenji Hata, Shiro Nakamura, Rika Yasuhara, Koki Takamatsu, Tarou Irie, Toshiyuki Fukada, Takayoshi Sakai, Tomio Inoue, Riko Nishimura, Osamu Ohara, Ichiro Saito, Shinsuke Ohba, Takashi Tsuji, Kenji Mishima (2018) Generation of orthotopically functional salivary gland from embryonic stem cells., Nature communications, Volume 9, Number 1, pp. 4216
Published in 2018 Oct 11 (Electronic publication in Oct. 11, 2018, midnight )
- doi: 10.1038/s41467-018-06469-7
-
pmid: 30310071
(Abstract) Organoids generated from pluripotent stem cells are used in the development of organ replacement regenerative therapy by recapitulating the process of organogenesis. These processes are strictly regulated by morphogen signalling and transcriptional networks. However, the precise transcription factors involved in the organogenesis of exocrine glands, including salivary glands, remain unknown. Here, we identify a specific combination of two transcription factors (Sox9 and Foxc1) responsible for the differentiation of mouse embryonic stem cell-derived oral ectoderm into the salivary gland rudiment in an organoid culture system. Following orthotopic transplantation into mice whose salivary glands had been removed, the induced salivary gland rudiment not only showed a similar morphology and gene expression profile to those of the embryonic salivary gland rudiment of normal mice but also exhibited characteristics of mature salivary glands, including saliva secretion. This study suggests that exocrine glands can be induced from pluripotent stem cells for organ replacement regenerative therapy.(MeSH Terms)
- Animals
- Cells, Cultured
- Ectoderm/metabolism
- Female
- Gene Expression Profiling
- HEK293 Cells
- Humans
- Mice
- Mice, Inbred C57BL
- Mouse Embryonic Stem Cells/cytology[major]
- Mouse Embryonic Stem Cells/metabolism
- Mouth Mucosa/embryology
- Mouth Mucosa/metabolism
- Salivary Glands/cytology
- Salivary Glands/growth & development[major]
- Salivary Glands/transplantation
- Salivary Glands/ultrastructure
- Transcription Factors/metabolism
Contact
Kenji Mishima
, Showa University
, Department of Oral Diagnostic Sciences, School of Dentistry
, Division of Pathology
Contributors
Junichi Tanaka, Miho Ogawa, Hironori Hojo, Yusuke Kawashima, Yo Mabuchi, Kenji Hata, Shiro Nakamura, Rika Yasuhara, Koki Takamatsu, Tarou Irié, Toshiyuki Fukada, Takayoshi Sakai, Tomio Inoue, Riko Nishimura, Osamu Ohara, Ichiro Saito, Shinsuke Ohba, Takashi Tsuji, Kenji Mishima
OMERO Dataset
OMERO Project
Source