Detail of Fig4F_photoreceptor
Project
Title
Electron microscopy image of mature photoreceptors inside the rosette-like structure derived from hESC retinal sheets
Description
mature photoreceptors by electron microscopy
Release, Updated
2019-11-20
License
CC BY-NC-SA
Kind
Image data
based on Experiment
File Formats
Data size
3.0 MB
Datatype
tissue morphology
Molecular Function (MF)
Biological Process (BP)
retinal development
(
GO:0060041
)
eye photoreceptor cell development
(
GO:0042462
)
Cellular Component (CC)
photoreceptor cell cilium
(
GO:0097733
)
Biological Imaging Method
XYZ Scale
XY: NA, Z: NA
T scale
-
Image Acquisition
Experiment type
Other
Microscope type
BrightfieldMicroscope
Acquisition mode
WideField
Contrast method
Brightfield
Microscope model
Electron microscope
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Iraha et al. (2018) Stem Cell Reports, 10(3): 1059-1074.
Related paper(s)
Satoshi Iraha, Hung-Ya Tu, Suguru Yamasaki, Takahiro Kagawa, Motohito Goto, Riichi Takahashi, Takehito Watanabe, Sunao Sugita, Shigenobu Yonemura, Genshiro A Sunagawa, Take Matsuyama, Momo Fujii, Atsushi Kuwahara, Akiyoshi Kishino, Naoshi Koide, Mototsugu Eiraku, Hidenobu Tanihara, Masayo Takahashi, Michiko Mandai (2018) Establishment of Immunodeficient Retinal Degeneration Model Mice and Functional Maturation of Human ESC-Derived Retinal Sheets after Transplantation., Stem cell reports, Volume 10, Number 3, pp. 1059-1074
Published in 2018 Mar 13 (Electronic publication in March 1, 2018, midnight )
- doi: 10.1016/j.stemcr.2018.01.032
-
pmid: 29503091
(Abstract) Increasing demand for clinical retinal degeneration therapies featuring human ESC/iPSC-derived retinal tissue and cells warrants proof-of-concept studies. Here, we established two mouse models of end-stage retinal degeneration with immunodeficiency, NOG-rd1-2J and NOG-rd10, and characterized disease progress and immunodeficient status. We also transplanted human ESC-derived retinal sheets into NOG-rd1-2J and confirmed their long-term survival and maturation of the structured graft photoreceptor layer, without rejection or tumorigenesis. We recorded light responses from the host ganglion cells using a multi-electrode array system; this result was consistent with whole-mount immunostaining suggestive of host-graft synapse formation at the responding sites. This study demonstrates an application of our mouse models and provides a proof of concept for the clinical use of human ESC-derived retinal sheets.(MeSH Terms)
- Animals
- Disease Models, Animal
- Embryonic Stem Cells/pathology[major]
- Female
- Humans
- Induced Pluripotent Stem Cells/pathology
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Inbred NOD
- Mice, Transgenic
- Photoreceptor Cells/pathology
- Retina/pathology[major]
- Retinal Degeneration/pathology[major]
- Stem Cell Transplantation/methods
Contact
Michiko Mandai
, RIKEN
, Center for Biosystems Dynamics Research
, Laboratory for Retinal Regeneration
Contributors
Satoshi Iraha, Hung-Ya Tu, Suguru Yamasaki, Takahiro Kagawa, Motohito Goto, Riichi Takahashi, Takehito Watanabe, Sunao Sugita, Shigenobu Yonemura, Genshiro A. Sunagawa, Take Matsuyama, Momo Fujii, Atsushi Kuwahara, Akiyoshi Kishino, Naoshi Koide, Mototsugu Eiraku, Hidenobu Tanihara, Masayo Takahashi, Michiko Mandai
OMERO Dataset
OMERO Project