Detail of Fig2b_E3.75_1



Project
SSBD:Repository
Title
Immunofluorescence confocal image of Xist/Tsix expression patterns in the mouse extraembryonic TE/ExE lineage cells at E3.75
Description
NA
Release, Updated
2019-11-20
License
CC-BY
Kind
Image data based on Experiment
File Formats
Data size
18.4 MB

Organism
M. musculus ( NCBITaxon:10090 )
Strain(s)
-
Cell Line
-
Gene symbols
Xist, Tsix

Datatype
X chromosome inactivation (XCI) dynamics
Molecular Function (MF)
Biological Process (BP)
gene expression ( GO:0010467 ) dosage compensation by inactivation of X chromosome ( GO:0009048 )
Cellular Component (CC)
necleus ( GO:0005634 )
Biological Imaging Method
XYZ Scale
XY: 0.1660532 micrometer/pixel, Z: 0.8 micrometer/slice
T scale
-

Image Acquisition
Experiment type
Immunofluorescence
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
See details in Shiura et al. (2019) Sci Rep, 9(1): 3637.
Related paper(s)

Hirosuke Shiura, Kuniya Abe (2019) Xist/Tsix expression dynamics during mouse peri-implantation development revealed by whole-mount 3D RNA-FISH., Scientific reports, Volume 9, Number 1, pp. 3637

Published in 2019 Mar 6 (Electronic publication in March 6, 2019, midnight )

(Abstract) During peri-implantation development in mice, X chromosome inactivation (XCI) status changes dynamically. Here, we examined the expression of Xist and its antisense partner, Tsix, via whole-mount 3D RNA-FISH using strand-specific probes and evaluated XCI status. The results indicate that Xist expression disappears completely by embryonic day (E) 4.5 without Tsix activation in the ICM and that Xist re-expression occurs at E4.75 in some cells, suggesting that random XCI is already initiated in these cells. Intriguingly, epiblast cells exhibiting biallelic Xist expression were observed frequently (~15%) at E5.25 and E5.5. Immunostaining analysis of epigenetic modifications suggests that global change in epigenomic status occurs concomitantly with the transition from imprinted to random XCI. However, global upregulation of H3K27me3 modifications initiated earlier than other modifications, occurring specifically in ICM during progression of Xist erasure. Although both Xist expression and imprinted XCI are thought to be stable in the primitive endoderm/visceral endoderm and trophectoderm/extraembryonic ectoderm lineages, transient loss of Xist clouds was noted only in a subset of extraembryonic ectodermal cells, suggesting distinct features of Xist regulation among the three different embryonic tissue layers. These results will serve as a basis for future functional studies of XCI regulation in vivo.
(MeSH Terms)

Contact
Kuniya Abe, Hirosuke Shiura , RIKEN , BioResource Research Center , Technology & Development Team for Mammalian Genome Dynamics,
Contributors
Hirosuke Shiura, Kuniya Abe

OMERO Dataset
OMERO Project
Source