Detail of fig1f_ERKactivation
Project
SSBD:Repository
Title
Time-lapse images of numerous polymorphonuclear cells (PMNs) infiltarting into the luns of tumor-bearing mice with ERK activation
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
105.7 MB
Organism
Mus musculus
(
NCBI:txid10090
)
Strain(s)
PMN expressing ERK biosensor and 4T1 tumor cells expressing Red fluorescent protein
Cell Line
-
Protein names
ERK
Datatype
Cell dynamics, ERK activation by FRET
Molecular Function (MF)
Biological Process (BP)
activation of MAPKK activity
(
GO:0007555
)
Cellular Component (CC)
cell
(
GO:0005623
)
Biological Imaging Method
XYZ Scale
XY: 0.552 micrometer/pixel, Z: 1 micrometer/slice
T scale
59.07 second for each time interval
Image Acquisition
Experiment type
FRET
Microscope type
MultiPhotonMicroscopy
Acquisition mode
-
Contrast method
Fluorescence
Microscope model
Olympus FV1200MPE-BX61WI
Detector model
-
Objective model
Olympus XLPLN 25XW-MP
Filter set
Summary of Methods
See details in Kamioka et al. (2017) Cancer Sci, 108(2): 226-235.
Related paper(s)
Contact
Yuji Kamioka
, Kansai Medical University
Contributors
Yuji Kamioka, Kanako Takakura, Kenta Sumiyama, Michiyuki Matsuda
OMERO Dataset
OMERO Project