Detail of fig1D_immuno
Project
Title
Immunocytochemistry image of ZIP10 (red) and E-cadherin (green) in skin at mouse postnatal day 2
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
4.1 MB
Protein names
ZIP10, E-cadherin
Datatype
immnocytochemistry of skin
Molecular Function (MF)
Biological Process (BP)
homeostasis
(
GO:0042592
)
zinc transport
(
GO:0006829
)
Cellular Component (CC)
-
Biological Imaging Method
XYZ Scale
XY: 0.645 micrometer/pixel, Z: NA
T scale
-
Image Acquisition
Experiment type
TimeLapse
Microscope type
FluorescenceMicroscope
Acquisition mode
FluorescenceCorrelationSpectroscopy
Contrast method
Fluorescence
Microscope model
Carl Zeiss LSM 700 or LSM780 or Axio Scan.Z1
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Bin et al. (2017) Proc Natl Acad Sci U S A, 114(46): 12243-12248.
Related paper(s)
Bum-Ho Bin, Jinhyuk Bhin, Mikiro Takaishi, Koh-Ei Toyoshima, Saeko Kawamata, Kana Ito, Takafumi Hara, Takashi Watanabe, Tarou Irie, Teruhisa Takagishi, Su-Hyon Lee, Haeng-Sun Jung, Sangchul Rho, Juyeon Seo, Dong-Hwa Choi, Daehee Hwang, Haruhiko Koseki, Osamu Ohara, Shigetoshi Sano, Takashi Tsuji, Kenji Mishima, Toshiyuki Fukada (2017) Requirement of zinc transporter ZIP10 for epidermal development: Implication of the ZIP10-p63 axis in epithelial homeostasis., Proceedings of the National Academy of Sciences of the United States of America, Volume 114, Number 46, pp. 12243-12248
Published in 2017 Nov 14 (Electronic publication in Oct. 23, 2017, midnight )
- doi: 10.1073/pnas.1710726114
-
pmid: 29078349
(Abstract) Skin tissues, in particular the epidermis, are severely affected by zinc deficiency. However, the zinc-mediated mechanisms that maintain the cells that form the epidermis have not been established. Here, we report that the zinc transporter ZIP10 is highly expressed in the outer root sheath of hair follicles and plays critical roles in epidermal development. We found that ZIP10 marked epidermal progenitor cell subsets and that ablating Zip10 caused significant epidermal hypoplasia accompanied by down-regulation of the transactivation of p63, a master regulator of epidermal progenitor cell proliferation and differentiation. Both ZIP10 and p63 are significantly increased during epidermal development, in which ZIP10-mediated zinc influx promotes p63 transactivation. Collectively, these results indicate that ZIP10 plays important roles in epidermal development via, at least in part, the ZIP10-zinc-p63 signaling axis, thereby highlighting the physiological significance of zinc regulation in the maintenance of skin epidermis.(MeSH Terms)
- Animals
- Cation Transport Proteins/genetics[major]
- Cation Transport Proteins/metabolism
- Cations, Divalent
- Cell Differentiation
- Cell Proliferation
- Embryo, Mammalian
- Gene Expression Profiling
- Gene Expression Regulation, Developmental
- Hair Follicle/growth & development
- Hair Follicle/metabolism[major]
- HeLa Cells
- Homeostasis/genetics[major]
- Humans
- Ion Transport
- Mice
- Mice, Transgenic
- Phosphoproteins/genetics[major]
- Phosphoproteins/metabolism
- Receptors, G-Protein-Coupled/genetics
- Receptors, G-Protein-Coupled/metabolism
- Signal Transduction
- Skin/cytology
- Skin/growth & development
- Skin/metabolism[major]
- Tissue Culture Techniques
- Trans-Activators/genetics[major]
- Trans-Activators/metabolism
- Zinc/metabolism[major]
Contact
Toshiyuki Fukada
, Showa University
, Division of Pathology, Department of Oral Diagnostic Sciences, School of Dentistry
Contributors
Bum-Ho Bin, Jinhyuk Bhin, Mikiro Takaishi, Koh-ei Toyoshima, Saeko Kawamata, Kana Ito, Takafumi Hara, Takashi Watanabe, Tarou Irié, Teruhisa Takagishi, Su-Hyon Lee, Haeng-Sun Jung, Sangchul Rho, Juyeon Seo, Dong-Hwa Choi, Daehee Hwang, Haruhiko Koseki, Osamu Ohara, Shigetoshi Sano, Takashi Tsuji, Kenji Mishima, Toshiyuki Fukada
OMERO Dataset
OMERO Project
Source