Detail of fig4E


Project
SSBD:Repository
Title
FRET image of ERK activity in HeLa cells
Description
NA
Release, Updated
2018-11-14
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
38.3 MB

Organism
Homo sapiens ( NCBI:txid9606 )
Strain(s)
HeLa
Cell Line
-
Protein names
ERK

Datatype
cell signaling dynamics
Molecular Function (MF)
Biological Process (BP)
intracellular signal transduction ( GO:0035556 )
Cellular Component (CC)
cell ( GO:0005623 )
Biological Imaging Method
XYZ Scale
XY:0.4919990 micrometer/pixel, Z:1 micrometer/slice
T scale
120 second for each time interval

Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus IX81 FV1000
Detector model
-
Objective model
Olympus UPLANSAPO 60xO/N.A. 1.35
Filter set
-

Summary of Methods
See details in Uda et al. (2017) Proc Natl Acad Sci U S A, 114(45): 11962-11967.
Related paper(s)

Contact
Kazuhiro Aoki , National Institutes of Natural Sciences , National Institute for Basic Biology , Division of Quantitative Biology
Contributors
Youichi Uda, Yuhei Goto, Shigekazu Oda, Takayuki Kohchi, Michiyuki Matsuda, Kazuhiro Aoki

OMERO Dataset
OMERO Project
Source