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Key
Organism
Strain
Cell Line
Gene Symbol
Protein name
Protein tag
Reporter
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Dataset ID
Title
Description
Data Type
Gene Ontology
Biological Imaging Methods
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Detail of fig2C_PhyB-PIF6_0012
SSBD:OMERO.gallery Dataset
SSBD:OMERO Dataset
SSBD:OMERO.gallery Project
SSBD:OMERO Project
4D View for Quantitative Data
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Project
92-Uda-CellsigDyn
SSBD:Repository
ssbd-repos-000092
Title
Fluorescence image of HeLa cells expressing PHFF, PhyB-mCherry-HRasCT and PIF6-mEGFP
Description
NA
Release, Updated
2018-11-14
License
CC BY
Kind
Image data based on Experiment
File Formats
Data size
58.7 MB
Organism
Homo sapiens
(
NCBI:txid9606
)
Strain(s)
HeLa
Cell Line
-
Protein names
PHFF, HRasCT, PIF6
Datatype
cell signaling dynamics
Molecular Function (MF)
Biological Process (BP)
intracellular signal transduction (
GO:0035556
)
Cellular Component (CC)
cell (
GO:0005623
)
Biological Imaging Method
XYZ Scale
XY:0.331 micrometer/pixel, Z:1 micrometer/slice
T scale
3.64 second for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus IX81 FV1000
Detector model
-
Objective model
Olympus UPLANSAPO 60xO/N.A. 1.35
Filter set
-
Summary of Methods
See details in Uda et al. (2017) Proc Natl Acad Sci U S A, 114(45): 11962-11967.
Related paper(s)
Contact
Kazuhiro Aoki , National Institutes of Natural Sciences , National Institute for Basic Biology , Division of Quantitative Biology
Contributors
Youichi Uda, Yuhei Goto, Shigekazu Oda, Takayuki Kohchi, Michiyuki Matsuda, Kazuhiro Aoki
OMERO Dataset
https://ssbd.riken.jp/omero/webclient/?show=dataset-928
OMERO Project
https://ssbd.riken.jp/omero/webclient/?show=project-165
Source
https://ssbd.riken.jp/data/92-Uda-CellsigDyn/source/fig2C_PhyB-PIF6_0012.zip
(31.8 MB)