Detail of fig1C_HeLa_AMPKAR_2DG
Project
SSBD:Repository
Title
FRET image of HeLa cells expressing AMPKAR stimulated with 10mM 2-deoxyglucose
Description
NA
Release, Updated
2018-11-14
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
1.0 GB
Datatype
Cell dynamic
Molecular Function (MF)
Biological Process (BP)
AMP activated protein kinase activity
(
GO:0004679
)
Cellular Component (CC)
nucleotide activated protein kinase complex
(
GO:0031588
)
Biological Imaging Method
XYZ Scale
XY: 0.454 micrometer/pixel, Z: one slice
T scale
3 minutes for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
FluorescenceMicroscope
Acquisition mode
Other
Contrast method
Fluorescence
Microscope model
Olympus FV1000MVE or Olympus FV1200MPE-BX61WI
Detector model
two-channel GaAsP and two multialkali
Objective model
UplanSApo 30x/1.05 silicon or UplanSApo 25x/1.05 numerical aperture
Filter set
Summary of Methods
See details in Konagaya et al. (2017) Cell Rep, 21(9): 2628-2638.
Related paper(s)
Contact
Kenta Terai
, Kyoto University
, Graduate School of Biostudies
, Laboratory of Bioimaging and Cell Signaling
Contributors
Yumi Konagaya, Kenta Terai, Yusuke Hirao, Kanako Takakura, Masamichi Imajo, Yuji Kamioka, Norio Sasaoka, Akira Kakizuka, Kenta Sumiyama, Tomoichiro Asano, Michiyuki Matsuda
OMERO Dataset
OMERO Project
Source