SSBD:database

Detail of fig1B_AMPKAREV

Yumi Konagaya, Kenta Terai, Yusuke Hirao, Kanako Takakura, Masamichi Imajo, Yuji Kamioka, Norio Sasaoka, Akira Kakizuka, Kenta Sumiyama, Tomoichiro Asano, Michiyuki Matsuda (2017) A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs., Cell reports, Volume 21, Number 9, pp. 2628-2638

Published in 2017 Nov 28

• doi: 10.1016/j.celrep.2017.10.113
• pmid: 29186696
  

(Abstract) AMP-activated protein kinase (AMPK), a master regulator of cellular metabolism, is a potential target for type 2 diabetes. Although extensive in vitro studies have revealed the complex regulation of AMPK, much remains unknown about the regulation in vivo. We therefore developed transgenic mice expressing a highly sensitive fluorescence resonance energy transfer (FRET)-based biosensor for AMPK, called AMPKAR-EV. AMPKAR-EV allowed us to readily examine the role of LKB1, a canonical stimulator of AMPK, in drug-induced activation and inactivation of AMPK in vitro. In transgenic mice expressing AMPKAR-EV, the AMP analog AICAR activated AMPK in muscle. In contrast, the antidiabetic drug metformin activated AMPK in liver, highlighting the organ-specific action of AMPK stimulators. Moreover, we found that AMPK was activated primarily in fast-twitch muscle fibers after tetanic contraction and exercise. These observations suggest that the AMPKAR-EV mouse will pave a way to understanding the heterogeneous responses of AMPK among cell types in vivo.

• AMP-Activated Protein Kinases/metabolism[major]
• Animals
• Biosensing Techniques/methods[major]
• Female
• Fluorescence Resonance Energy Transfer/methods[major]
• Liver/metabolism
• Male
• Mice
• Muscle, Skeletal/metabolism
• Signal Transduction/genetics
• Signal Transduction/physiology