Detail of fig1E_BSA_0min
Project
Title
Images of BSA-treated neurons showed phosphorylation and nuclear translocation of Smad1/5/8 at 0 minute
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
2.6 MB
Protein names
Smad1/5/8
Datatype
synapse dynamics
Molecular Function (MF)
Biological Process (BP)
synaptogenesis
(
GO:0007416
)
neuromuscular junction
(
GO:0031594
)
Cellular Component (CC)
spine synapse
(
GO:0106033
)
Biological Imaging Method
XYZ Scale
XY: 0.225 micrometer/pixel, Z: NA
T scale
-
Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus FluoView FV10i
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
See details in Higashi et al. (2018) Cell Rep, 22(4): 919-929.
Related paper(s)
Takahito Higashi, Shinji Tanaka, Tadatsune Iida, Shigeo Okabe (2018) Synapse Elimination Triggered by BMP4 Exocytosis and Presynaptic BMP Receptor Activation., Cell reports, Volume 22, Number 4, pp. 919-929
Published in 2018 Jan 23 (Electronic publication in Jan. 28, 2018, midnight )
- doi: 10.1016/j.celrep.2017.12.101
-
pmid: 29386134
(Abstract) In vitro screening of signaling molecules involved in neural circuit formation has identified a large number of synaptogenic proteins. However, factors that drive synapse elimination remain elusive. Here, we report that bone morphogenetic protein 4 (BMP4) released from axons has the ability to eliminate synapses. We found fast axonal transport of BMP4 in dense-core vesicles, its exocytosis, and subsequent cell surface clustering via type I BMP receptors near synapses. BMP4 overexpression or knockout in culture reduced or increased presynaptic structures, respectively. The destabilizing effect of surface BMP4 clusters was limited to nearby synapses. In vivo knockout of BMP4 and subsequent two-photon imaging of synapse dynamics confirmed its critical role in maintaining an appropriate density of presynaptic components along the axon. These results suggest an essential role for perisynaptic clustering of BMP4 during development in the construction of functional neuronal circuits.(MeSH Terms)
Contact
Shigeo Okabe
, University of Tokyo
, Department of Cellular Neurobiology, Graduate School of Medicine
Contributors
Takahito Higashi, Shinji Tanaka, Tadatsune Iida, Shigeo Okabe
OMERO Dataset
OMERO Project