Detail of fig5e
Project
SSBD:Repository
Title
Time-lapse images of MLC2-EGFP expressed in HT29 cells during nocodazole treatment
Description
NA
Release, Updated
2018-11-14
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
37.4 MB
Protein names
MLC2
Protein tags
EGFP
Datatype
cell dynamics
Molecular Function (MF)
Biological Process (BP)
-
Cellular Component (CC)
apical junction complex
(
GO:0043296
)
Biological Imaging Method
XYZ Scale
XY: 353 micrometer/pixel, Z: NA micrometer/slice
T scale
120 second for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
DIC
Microscope model
Olympus LCV100 or Olympus IX81 + Yokogawa CSU-W1
Detector model
Olympus DP30 or ImagEM-IK
Objective model
Olympus UAPOx40/340 or PLANAPO N x60/1.42 oil lens
Filter set
Summary of Methods
See details in Ito et al. (2017) Nat Commun, 8(1): 1834-1849.
Related paper(s)
Contact
Masatoshi Takeichi
, RIKEN
, Center for Biosystems Dynamics Research
, Laboratory for Cell Adhesion and Tissue Patterning
Contributors
Shoko Ito, Satoru Okuda, Masako Abe, Mari Fujimoto, Tetsuo Onuki, Tamako Nishimura, Masatoshi Takeichi
OMERO Dataset
OMERO Project
Source