Detail of RNAi_B0336.10_040518_02
Project
Title
3D time-lapse microscopy images about nuclear division dynamics in B0336.10(RNAi) embryo (rpl-23(RNAi) embryo)
Description
NA
Release, Updated
2013-09-02,
2020-02-03
2020-02-03
License
CC BY-SA
Kind
Image data
based on Experiment
related Quantitative data ;
RNAi_B0336.10_040518_02
File Formats
Data size
8.0 GB
Gene symbols
B0336.10, rpl-23
Datatype
nuclear division dynamics
Molecular Function (MF)
Biological Process (BP)
embryo development
(
GO:0009790
)
Cellular Component (CC)
nucleus
(
GO:0005634
)
Biological Imaging Method
XYZ Scale
XY: 0.105 micrometer/pixel, Z: 0.5 micrometer/slice
T scale
40 second for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
DifferentInterferenceContrastMicroscope
Acquisition mode
-
Contrast method
DIC
Microscope model
Leica DMRE
Detector model
Hamamatsu Photonics ORCA C4742-95
Objective model
Leica HCX PL Apo 100x/1.40 O
Filter set
-
Summary of Methods
See details in Kyoda et al. (2013) Nucleic Acids Res 41, D732-D737
Related paper(s)
Koji Kyoda, Eru Adachi, Eriko Masuda, Yoko Nagai, Yoko Suzuki, Taeko Oguro, Mitsuru Urai, Ryoko Arai, Mari Furukawa, Kumiko Shimada, Junko Kuramochi, Eriko Nagai, Shuichi Onami (2013) WDDD: Worm Developmental Dynamics Database., Nucleic acids research, Volume 41, Number Database issue, pp. D732-7
Published in 2013 Jan (Electronic publication in Nov. 20, 2012, midnight )
- doi: 10.1093/nar/gks1107
-
pmid: 23172286
(Abstract) During animal development, cells undergo dynamic changes in position and gene expression. A collection of quantitative information about morphological dynamics under a wide variety of gene perturbations would provide a rich resource for understanding the molecular mechanisms of development. Here, we created a database, the Worm Developmental Dynamics Database (http://so.qbic.riken.jp/wddd/), which stores a collection of quantitative information about cell division dynamics in early Caenorhabditis elegans embryos with single genes silenced by RNA-mediated interference. The information contains the three-dimensional coordinate values of the outlines of nuclear regions and the dynamics of the outlines over time. The database provides free access to 50 sets of quantitative data for wild-type embryos and 136 sets of quantitative data for RNA-mediated interference embryos corresponding to 72 of the 97 essential embryonic genes on chromosome III. The database also provides sets of four-dimensional differential interference contrast microscopy images on which the quantitative data were based. The database will provide a novel opportunity for the development of computational methods to obtain fresh insights into the mechanisms of development. The quantitative information and microscopy images can be synchronously viewed through a web browser, which is designed for easy access by experimental biologists.(MeSH Terms)
Contact
Shuichi Onami
, RIKEN
, Quantitative Biology Center
, Laboratory for Developmental Dynamics
Contributors
Eru Adachi, Eriko Masuda, Yoko Nagai, Yoko Suzuki, Taeko Oguro, Koji Kyoda, Shuichi Onami
OMERO Dataset
OMERO Project
Source