Detail of Fig4E-G-S3H_54
Project
SSBD:Repository
Title
Image of sniff-alinged singe-trial responses of glomeruli
Description
NA
Release, Updated
2019-11-20
License
CC BY
Kind
Image data
based on Experiment
File Formats
Data size
32.7 MB
Datatype
calcium imaging data
Molecular Function (MF)
Biological Process (BP)
neuron cell to cell adhesion
(
GO:0007158
)
Cellular Component (CC)
axon
(
GO:0030424
)
dendrite
(
GO:0030425
)
Biological Imaging Method
XYZ Scale
XY: 1.988 micrometer/pixel, Z: NA
T scale
0.04 second for each time interval
Image Acquisition
Experiment type
TimeLapse
Microscope type
ConfocalMicroscope
Acquisition mode
LaserScanningConfocalMicroscopy
Contrast method
Fluorescence
Microscope model
Olympus FV1000MPE
Detector model
-
Objective model
Olympus XLPLN25XWMP
Filter set
-
Summary of Methods
See details in Iwata et al. (2017) Neuron, 96(5): 1139-1152.
Related paper(s)
Ryo Iwata, Hiroshi Kiyonari, Takeshi Imai (2017) Mechanosensory-Based Phase Coding of Odor Identity in the Olfactory Bulb., Neuron, Volume 96, Number 5, pp. 1139-1152.e7
Published in 2017 Dec 6
- doi: 10.1016/j.neuron.2017.11.008
-
pmid: 29216451
-
url: https://www.cell.com/neuron/fulltext/S0896-6273(17)31036-X
(Abstract) Mitral and tufted (M/T) cells in the olfactory bulb produce rich temporal patterns of activity in response to different odors. However, it remains unknown how these temporal patterns are generated and how they are utilized in olfaction. Here we show that temporal patterning effectively discriminates between the two sensory modalities detected by olfactory sensory neurons (OSNs): odor and airflow-driven mechanical signals. Sniff-induced mechanosensation generates glomerulus-specific oscillatory activity in M/T cells, whose phase was invariant across airflow speed. In contrast, odor stimulation caused phase shifts (phase coding). We also found that odor-evoked phase shifts are concentration invariant and stable across multiple sniff cycles, contrary to the labile nature of rate coding. The loss of oscillatory mechanosensation impaired the precision and stability of phase coding, demonstrating its role in olfaction. We propose that phase, not rate, coding is a robust encoding strategy of odor identity and is ensured by airflow-induced mechanosensation in OSNs.(MeSH Terms)
Contact
Takeshi Imai
, Kyushu University
, Graduate School of Medical Sciences
, Department of Developmental Neurophysiology
Contributors
Ryo Iwata, Hiroshi Kiyonari, Takeshi Imai
OMERO Dataset
OMERO Project