Detail of Fig6j_5cm_12cm_DARDAF


Project
SSBD:Repository
Title
Z-stack images of dermal collagen formation during growth from 5 to 12 centimeter body length in an axolotl
Description
The accumulation of collagen on the apical side of the dermis during growth from 5 to 12 centimeter body length was observed in Z-stack images acquired by confocal microscopy. DAR-4M was used as a fluorescent probe to visualize dermal collagen present at the beginning of the pulse-chase experiment and is referred to as DAR in this study. DAF-FM DA was subsequently applied to visualize collagen labeled at the later growth stage and is referred to as DAF in this study.
Release, Updated
2026-10-01
License
CC BY 4.0
Kind
Image data
File Formats
oir
Data size
321.3 MB

Organism
Ambystoma mexicanum ( NCBI:txid8296 )
Strain(s)
-
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
extracellular matrix organization collagen metabolic process collagen fibril organization collagen biosynthetic process
Cellular Component (CC)
extracellular matrix fibrillar collagen complex
Biological Imaging Method
confocal microscopy ( Fbbi:00000251 )
X scale
0.265 micrometer
Y scale
0.265 micrometer
Z scale
1.5 micrometer
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Ohashi A, Sakamoto H, Kuroda J, Kondo Y, Kamei Y, Nonaka S, Furukawa S, Yamamoto S, Satoh A. Keratinocyte-driven dermal collagen formation in the axolotl skin. Nat Commun. 2025 Feb 24;16(1):1757.
Related paper(s)

Contact
Akira Satoh , Okayama University , Faculty of Environmental, Life, Natural Science and Technology , Faculty of Environmental, Life, Natural Science and Technology
Contributors

OMERO Dataset
OMERO Project
Source