Detail of Fig3i_12cm_DAR_GFP


Project
SSBD:Repository
Title
Z-stack images of the mature dermal collagen structure in a 12 centimeter long axolotl
Description
The mature dermal collagen structure in the 12 centimeter long axolotl, consisting of the stratum baladachinum (SB), stratum spongiosum (SS), and stratum compactum (SC), was visualized in Z-stack images acquired by confocal microscopy. Cells were abundant in the SS. DAR-4M was used as a fluorescent probe for dermal collagen visualization and is referred to as DAR in this study. In the CMV::GFP transgenic axolotl, GFP fluorescence was used to visualize the cell cytoplasm and filopodia.
Release, Updated
2026-10-01
License
CC BY 4.0
Kind
Image data
File Formats
oir
Data size
153.6 MB

Organism
Ambystoma mexicanum ( NCBI:txid8296 )
Strain(s)
-
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
extracellular matrix organization collagen metabolic process collagen fibril organization
Cellular Component (CC)
extracellular matrix cytoplasm
Biological Imaging Method
confocal microscopy ( Fbbi:00000251 )
X scale
0.133 micrometer
Y scale
0.133 micrometer
Z scale
0.74 micrometer
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Ohashi A, Sakamoto H, Kuroda J, Kondo Y, Kamei Y, Nonaka S, Furukawa S, Yamamoto S, Satoh A. Keratinocyte-driven dermal collagen formation in the axolotl skin. Nat Commun. 2025 Feb 24;16(1):1757.
Related paper(s)

Contact
Akira Satoh , Okayama University , Faculty of Environmental, Life, Natural Science and Technology , Faculty of Environmental, Life, Natural Science and Technology
Contributors

OMERO Dataset
OMERO Project
Source