Detail of Fig3c_8cm_DAR_GFP
Project
SSBD:Repository
Title
Z-stack images of mesenchymal cells into the collagen layer in an 8 centimeter long axolotl
Description
The mesenchymal cells invading the collagen layer in the dermis of an 8 centimeter long axolotl were visualized in Z-stack images acquired by confocal microscopy. The filopodia extending from the mesenchymal cells were also observed. DAR-4M was used as a fluorescent probe for dermal collagen visualization and is referred to as DAR in this study. In the CMV::GFP transgenic axolotl, GFP fluorescence was used to visualize the cell cytoplasm and filopodia.
Release, Updated
2026-10-01
License
CC BY 4.0
Kind
Image data
File Formats
oir
Data size
91.8 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
extracellular matrix organization collagen metabolic process mesenchymal cell migration
Cellular Component (CC)
extracellular matrix cytoplasm
Biological Imaging Method
confocal microscopy
(
Fbbi:00000251
)
X scale
0.133 micrometer
Y scale
0.133 micrometer
Z scale
1.5 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ohashi A, Sakamoto H, Kuroda J, Kondo Y, Kamei Y, Nonaka S, Furukawa S, Yamamoto S, Satoh A. Keratinocyte-driven dermal collagen formation in the axolotl skin. Nat Commun. 2025 Feb 24;16(1):1757.
Related paper(s)
Contact
Akira Satoh
, Okayama University
, Faculty of Environmental, Life, Natural Science and Technology
, Faculty of Environmental, Life, Natural Science and Technology
Contributors
OMERO Project