Detail of Fig1_12cm_DAF_brightZ


Project
SSBD:Repository
Title
Z-stack images of type I collagen in the dorsal skin from a 12 centimeter long axolotl
Description
Type I collagen in the dorsal skin from a 12 centimeter long axolotl was visualized in the Z-stack images acquired by confocal microscopy. Three distinct collagen layers containing type I collagen were observed in the axolotl dermis using the fluorescent collagen probe DAF-FM DA. DAF-FM DA was used as a fluorescent probe for dermal collagen visualization and is referred to as DAF in this study.
Release, Updated
2026-10-01
License
CC BY 4.0
Kind
Image data
File Formats
oir
Data size
335.7 MB

Organism
Ambystoma mexicanum ( NCBI:txid8296 )
Strain(s)
-
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
extracellular matrix organization collagen metabolic process collagen fibril organization
Cellular Component (CC)
extracellular matrix
Biological Imaging Method
confocal microscopy ( Fbbi:00000251 )
X scale
0.414 micrometer
Y scale
0.414 micrometer
Z scale
0.74 micrometer
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Ohashi A, Sakamoto H, Kuroda J, Kondo Y, Kamei Y, Nonaka S, Furukawa S, Yamamoto S, Satoh A. Keratinocyte-driven dermal collagen formation in the axolotl skin. Nat Commun. 2025 Feb 24;16(1):1757.
Related paper(s)

Contact
Akira Satoh , Okayama University , Faculty of Environmental, Life, Natural Science and Technology , Faculty of Environmental, Life, Natural Science and Technology
Contributors

OMERO Dataset
OMERO Project
Source