Detail of Fig9a_AfConv_3D
SSBD:Repository
Title
Z-series images of x-y cross-sectional EpH4-spheroids expressing nucleus-localized PSmOrange2 after photostimulation of target cells
Description
Z-series images of a living EpH4 cell spheroid expressing nucleus-localized PSmOrange2, acquired after photoconversion through two-photon laser-scanning microscopy system (2P-LSM). The cells were photostimulated using the TM-ML-TF-CGH system. TM-ML-TF is short for time-multiplexed multiline temporal focusing. CGH is short for computer-generated holography. The spheroids were from living mouse mammary epithelial EpH4 cells expressing PSmOrange2. EpH4 cells are a spontaneously immortalized, non-tumorigenic mouse mammary epithelial cell line. PSmOrange2 here is a monomeric orange-to-far-red photoconvertible fluorescent protein used for optical labeling and tracking of selected EpH4 cells through photoconversion. The images before photoconversion were included in the image dataset: Fig9a_BfConv_3D.
Release, Updated
2026-10-01
License
CC BY 4.0
Kind
Image data
File Formats
tif
Data size
40.0 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
cell growth
Cellular Component (CC)
nucleus
Biological Imaging Method
two-photon laser scanning microscopy
(
Fbbi:00000254
)
X scale
0.586 micrometer
Y scale
0.586 micrometer
Z scale
1.0 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Inazawa K, Yamada M, Michikawa T, Namiki K, Miyawaki A, Imayoshi I, Midorikawa K, Isobe K. Three-dimensional scanless patterned illumination using time-multiplexed multiline temporal focusing for multicell manipulation with single-cell resolution. J Biomed Opt. 2025 Jul;30(7):075003.
Related paper(s)
Contact
Keisuke Isobe
, RIKEN RAP
Contributors
OMERO Project
Source