Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in control Drosophila pupa
Description
Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in control Drosophila pupa from 20 hours after puparium formation (hAPF) to 40 hAPF. The nuclei of larval epidermal cells were specifically visualized by the nls::CFP::Venus expressed under the control of pnr-GAL4. In the larval epidermal cells, miniCic::mScarlet (an ERK signaling reporter, miniCic, tagged with mScarlet) was expressed. For temperature sensitive experiments, the pupa was incubated at 18 degC for 24 h then 29 degC for 6 h.
Yuswan K, Sun X, Kuranaga E, Umetsu D. Reduction of endocytosis and EGFR signaling is associated with the switch from isolated to clustered apoptosis during epithelial tissue remodeling in Drosophila. PLoS Biology. 2024;22(10):e3002823.
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Erina Kuranaga, Daiki Umetsu
, Kyoto University, Osaka University
, Graduate School and Faculty of Pharmaceutical Sciences, Department of Biological Sciences, Graduate School of Science
, Graduate School and Faculty of Pharmaceutical Sciences, Department of Biological Sciences, Graduate School of Science