Detail of Fig2E_LEC_ERKsignal_Rab5active
Project
SSBD:Repository
Title
Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in Rab5-Q88L Drosophila pupae.
Description
Time-lapse images of miniCic::mScarlet and nls::CFP::Venus in Rab5-Q88L Drosophila pupa from 20 hours after puparium formation (hAPF) to 35 hAPF. The nuclei of larval epidermal cells were specifically visualized by the nls::CFP::Venus expressed under the control of pnr-GAL4. In the larval epidermal cells, miniCic::mScarlet (an ERK signaling reporter, miniCic, tagged with mScarlet) was expressed. Constitutively active form of the endosome internalization regulator Rab5 GTPase (Rab5-Q88L) was also expressed in the cells.
Release, Updated
2026-10-01
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
7.3 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
pupal development programmed cell death tissue morphogenesis
Cellular Component (CC)
nucleus cytosol
Biological Imaging Method
confocal microscopy
(
Fbbi:00000251
)
X scale
0.57 micrometer
Y scale
0.57 micrometer
Z scale
2.5 micrometer
T scale
10 minutes
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Yuswan K, Sun X, Kuranaga E, Umetsu D. Reduction of endocytosis and EGFR signaling is associated with the switch from isolated to clustered apoptosis during epithelial tissue remodeling in Drosophila. PLoS Biology. 2024;22(10):e3002823.
Related paper(s)
Contact
Erina Kuranaga, Daiki Umetsu
, Kyoto University, Osaka University
, Graduate School and Faculty of Pharmaceutical Sciences, Department of Biological Sciences, Graduate School of Science
, Graduate School and Faculty of Pharmaceutical Sciences, Department of Biological Sciences, Graduate School of Science
Contributors
OMERO Project
Source