Detail of Fig7_bf_dead
Project
SSBD:Repository
Title
Bright-field images of dead tobacco BY-2 cells
Description
Bright-field images for random forest classification of cell viability. Tobacco BY-2 cells were treated with 10 micromolar enoxolone for 24 h at 27 degC in the dark with shaking and imaged using an Agilent BioTek Cytation 1. ImageJ was used to manually crop 1,200 individual dead cells; 900 images were used for training and 300 for testing. GLCM texture features were extracted using ImageJ LPX Features and classified using the R package randomForest.
Release, Updated
2026-09-30
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
23.1 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
Transmitted bright-field microscopy
X scale
0.56 micrometer
Y scale
0.56 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ichita M, Yamamichi H, Higaki T. Virtual staining from bright-field microscopy for label-free quantitative analysis of plant cell structures. Plant Molecular Biology. 2025;115(1):29.
Related paper(s)
Contact
Takumi Higaki
, Kumamoto University
, Graduate School of Science and Technology
, Graduate School of Science and Technology
Contributors
OMERO Project