Detail of SupplFigS1_BY2_BCECF
Project
SSBD:Repository
Title
Bright-field and confocal images of wild-type tobacco BY-2 cells stained with BCECF for vacuolar lumen visualization
Description
This dataset was acquired to establish the deep-learning model for the virtual staining of plant cell structures. It includes images of wild-type tobacco BY-2 cells stained with BCECF (green) to visualize the vacuolar lumen. The file consists of 175 frames, of which the first 150 frames were used for training and the last 25 frames for testing. The stacked images combine bright-field and fluorescence microscopy, allowing for detailed vacuole structure analysis using virtual staining techniques. This dataset is the same as the one registered on Figshare. https://doi.org/10.6084/m9.figshare.27247638.v1
Release, Updated
2026-09-30
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
2.7 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
vacuole
Biological Imaging Method
spinning disk confocal microscopy
(
Fbbi:00000253
)
X scale
0.56 micrometer
Y scale
0.56 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ichita M, Yamamichi H, Higaki T. Virtual staining from bright-field microscopy for label-free quantitative analysis of plant cell structures. Plant Molecular Biology. 2025;115(1):29.
Related paper(s)
Contact
Takumi Higaki
, Kumamoto University
, Graduate School of Science and Technology
, Graduate School of Science and Technology
Contributors
OMERO Project
Source