Detail of FigS4B_Nidopallium_Right_YFP
Project
SSBD:Repository
Title
Z-series images of a cleared right brain of zebra finch showing tutor’s song responsive neurons in NCM
Description
Z-series images obtained with a custom-made light-sheet fluorescence microscope. The EYFP expressing neurons were immunohistochemically enhanced with anti-GFP antibody. This dataset includes the images for EYFP.
Release, Updated
2026-08-19
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
22.6 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
regulation of nervous system development
Cellular Component (CC)
cytoplasm
Biological Imaging Method
light-sheet microscopy
X scale
4.1 micrometer
Y scale
4.1 micrometer
Z scale
4.1 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
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Filter set
-
Summary of Methods
Louder MIM, Kuroda M, Taniguchi D, Komorowska-Muller JA, Morohashi Y, Takahashi M, Sanchez-Valpuesta M, Wada K, Okada Y, Hioki H, Yazaki-Sugiyama Y. Transient sensorimotor projections in the developmental song learning period. Cell Rep. 2024 May 28;43(5):114196.
Related paper(s)
Matthew I M Louder, Masafumi Kuroda, Daisuke Taniguchi, Joanna Agnieszka Komorowska-Muller, Yuichi Morohashi, Megumu Takahashi, Miguel Sanchez-Valpuesta, Kazuhiro Wada, Yasushi Okada, Hiroyuki Hioki, Yoko Yazaki-Sugiyama (2024) Transient sensorimotor projections in the developmental song learning period., Cell reports, Volume 43, Number 5, pp. 114196
Published in 2024 May 7 (Electronic publication in May 7, 2024, midnight )
- doi: 10.1016/j.celrep.2024.114196
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pmid: 38717902
(Abstract) Memory recall and guidance are essential for motor skill acquisition. Like humans learning to speak, male zebra finches learn to sing by first memorizing and then matching their vocalization to the tutor's song (TS) during specific developmental periods. Yet, the neuroanatomical substrate supporting auditory-memory-guided sensorimotor learning has remained elusive. Here, using a whole-brain connectome analysis with activity-dependent viral expression, we identified a transient projection into the motor region, HVC, from neuronal ensembles responding to TS in the auditory forebrain, the caudomedial nidopallium (NCM), in juveniles. Virally induced cell death of the juvenile, but not adult, TS-responsive NCM neurons impaired song learning. Moreover, isolation, which delays closure of the sensory, but not the motor, learning period, did not affect the decrease of projections into the HVC from the NCM TS-responsive neurons after the song learning period. Taken together, our results suggest that dynamic axonal pruning may regulate timely auditory-memory-guided vocal learning during development.
Contact
Yoko Yazaki-Sugiyama
, The University of Tokyo
, International Research Center for Neurointelligence (WPI-IRCN)
, International Research Center for Neurointelligence (WPI-IRCN)
Contributors
OMERO Project
Source