Detail of FigS1J_mutKirOver_3
Project
SSBD:Repository
Title
Z-series images of mouse olfactory bulb with overexpression of mutant-Kir2.1 in mitral cells
Description
Z-series immunostaining images of P6 mouse brain. By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato) and a small amount of Cre plasmid (pCAG-Cre) at E12 to label a subset of mitral cells sparsely. pCALNL-mutKir2.1 was also elactroporated to overexpress mutant Kir2.1. The mutant dose not conduct potassium ion. The brain was sliced and cleared with SeeDB2, and staind for Kir2.1 and DAPI.
Release, Updated
2026-08-07
License
CC BY 4.0
Kind
Image data
File Formats
.oib
Data size
607.8 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
neuron development neuron remodeling
Cellular Component (CC)
cell body dendrite
Biological Imaging Method
two-photon laser scanning microscopy
(
Fbbi:00000254
)
X scale
0.497 micrometer
Y scale
0.497 micrometer
Z scale
10 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T. Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Dev Cell. 2023 Jul 24;58(14):1221-1236.e7.
Related paper(s)
Contact
Takeshi Imai,
, Kyushu University,
, Graduate School of Medical Sciences,
, Graduate School of Medical Sciences,
Contributors
OMERO Project