Detail of FigS1I_GluSynapse_KirOver
Project
SSBD:Repository
Title
Z-series images of Kir2.1-overexpressing mitral cells and glutamatergic synapses in P6 mouse olfactory bulb
Description
Z-series images of mitral cells and post synapses in P6 mouse olfactory bulb with overexpression of Kir2.1. By using in utero electroporation, the authors electroporated a Cre-dependent tdTomato plasmid (pCALNL-tdTomato) and a small amount of Cre plasmid (pCAG-Cre) at E12 to label a subset of mitral cells sparsely. pCALNL-Kir2.1 was also elactroporated to overexpress Kir2.1, and reduce the activity of mitral cells. PSD d1.2-GFP was also expressed to visualize glutamatergic synapses.
Release, Updated
2026-08-07
License
CC BY 4.0
Kind
Image data
File Formats
.lif
Data size
476.1 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
dendrite glutamatergic synapse
Biological Imaging Method
two-photon laser scanning microscopy
(
Fbbi:00000254
)
X scale
0.06 micrometer
Y scale
0.06 micrometer
Z scale
0.12 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T. Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Dev Cell. 2023 Jul 24;58(14):1221-1236.e7.
Related paper(s)
Contact
Takeshi Imai,
, Kyushu University,
, Graduate School of Medical Sciences,
, Graduate School of Medical Sciences,
Contributors
OMERO Project
Source