Detail of Fig7_BarrelCortex_Grin1KO_RhoA_2
Project
SSBD:Repository
Title
Z-series images of the barrel cortex of Grin1-KO and RhoA-overexpressed mouse at P14 in which layer 4 neurons were labeled
Description
Z-series images of the barrel cortex of Grin1-KO and RhoA-overexpressed mouse at P14 in which layer 4 neurons were labeled by tdTomato. Grin1 coding NR1, an essential subunit of NMDA receptor was knocked out by using CRISPR-Cas9 and in utero electroporation. In this experoment, CRISPR-resistant-pCAG-RhoA was also introduced by in utero electroporation. VGluT2 was visualized by immmunohistochemistry.
Release, Updated
2026-08-07
License
CC BY 4.0
Kind
Image data
File Formats
.lif
Data size
52.3 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
neuron development neuron remodeling
Cellular Component (CC)
cell body dendrite
Biological Imaging Method
confocal microscopy
(
Fbbi:00000251
)
X scale
0.284 micrometer
Y scale
0.284 micrometer
Z scale
1.04 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T. Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Dev Cell. 2023 Jul 24;58(14):1221-1236.e7.
Related paper(s)
Contact
Takeshi Imai,
, Kyushu University,
, Graduate School of Medical Sciences,
, Graduate School of Medical Sciences,
Contributors
OMERO Project
Source