Detail of Fig5G_Raichu-Rac1_NMDA1uM_6
Project
SSBD:Repository
Title
FRET imaging of Rac1 activity in mitral cells after 1uM NMDA stimulation using the RaichuEV-Rac1 biosensor in the presence of TTX
Description
Time-lapse images of Rac1 activity in olfactory bulb slice from P3 mouse. To stimurate NMDA receptors, the slice was treated with 1 micromolar NMDA and glycine during imaging. Rac1 activity was studied with RaichuEV-Rac1, a FRET biosensor for Rac1.
Release, Updated
2026-08-07
License
CC BY 4.0
Kind
Image data
File Formats
.oib
Data size
406.0 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
postsynaptic signal transduction
Cellular Component (CC)
cell body dendrite
Biological Imaging Method
two-photon laser scanning microscopy
(
Fbbi:00000254
)
X scale
0.497 micrometer
Y scale
0.497 micrometer
Z scale
-
T scale
5 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T. Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Dev Cell. 2023 Jul 24;58(14):1221-1236.e7.
Related paper(s)
Contact
Takeshi Imai,
, Kyushu University,
, Graduate School of Medical Sciences,
, Graduate School of Medical Sciences,
Contributors
OMERO Project
Source