Detail of Fig5G_DORA-RhoA_NMDA20uM_2
Project
SSBD:Repository
Title
FRET imaging of RhoA activity in mitral cells after 20uM NMDA stimulation using the DORA-RhoA biosensor in the presence of TTX
Description
Time-lapse images of RhoA activity in olfactory bulb slice from P3 mouse. To stimurate NMDA receptors, the slice was treated with 20 micromolar NMDA and glycine during imaging. RhoA activity was studied with DORA-RhoA, a FRET biosensor for RhoA.
Release, Updated
2026-08-07
License
CC BY 4.0
Kind
Image data
File Formats
.oib
Data size
577.9 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
postsynaptic signal transduction
Cellular Component (CC)
cell body dendrite
Biological Imaging Method
two-photon laser scanning microscopy
(
Fbbi:00000254
)
X scale
0.497 micrometer
Y scale
0.497 micrometer
Z scale
-
T scale
5 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T. Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Dev Cell. 2023 Jul 24;58(14):1221-1236.e7.
Related paper(s)
Contact
Takeshi Imai,
, Kyushu University,
, Graduate School of Medical Sciences,
, Graduate School of Medical Sciences,
Contributors
OMERO Project
Source