Detail of Fig5ABC_mutDORA-RhoA_NMDA_1
Project
SSBD:Repository
Title
FRET imaging of RhoA activity in mitral cells after NMDA stimulation using the mutDORA-RhoA in the presence of TTX
Description
Time-lapse images of RhoA activity in olfactory bulb slice from P3 mouse. To stimurate NMDA receptors, the slice was treated with NMDA and glycine during imaging. RhoA activity was studied with mutDORA-RhoA, a mutant FRET sensor lacking RhoA binding activity.
Release, Updated
2026-08-07
License
CC BY 4.0
Kind
Image data
File Formats
.oib
Data size
410.5 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
postsynaptic signal transduction
Cellular Component (CC)
cell body dendrite
Biological Imaging Method
two-photon laser scanning microscopy
(
Fbbi:00000254
)
X scale
1.24 micrometer
Y scale
1.24 micrometer
Z scale
-
T scale
5 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T. Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Dev Cell. 2023 Jul 24;58(14):1221-1236.e7.
Related paper(s)
Contact
Takeshi Imai,
, Kyushu University,
, Graduate School of Medical Sciences,
, Graduate School of Medical Sciences,
Contributors
OMERO Project
Source