Detail of Fig4I_Grin1KO_RhoA_3


Project
SSBD:Repository
Title
Z-series images of Grin1-knockout and RhoA-overexpressed mouse brain at P6 in which mitral cells were labed by tdTomato
Description
Z-series images of Grin1 knockout mouse brain at P6 in which mitral cells were labed by tdTomato. Grin1 coding NR1, an essential subunit of NMDA receptor was knocked out by using CRISPR-Cas9 and in utero electroporation. In this experoment, CRISPR-resistant-pCAG-RhoA was also introduced by in utero electroporation.
Release, Updated
2026-08-07
License
CC BY 4.0
Kind
Image data
File Formats
.lif
Data size
1.8 GB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
ICR
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
neuron development neuron remodeling
Cellular Component (CC)
cell body dendrite
Biological Imaging Method
confocal microscopy ( Fbbi:00000251 )
X scale
0.568 micrometer
Y scale
0.569 micrometer
Z scale
5 micrometer
T scale
-

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T. Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Dev Cell. 2023 Jul 24;58(14):1221-1236.e7.
Related paper(s)

Contact
Takeshi Imai, , Kyushu University, , Graduate School of Medical Sciences, , Graduate School of Medical Sciences,
Contributors

OMERO Dataset
OMERO Project
Source