Detail of Fig4H_RhoABC-KO_RhoA_3
Project
SSBD:Repository
Title
Z-series images of RhoABC-knockout and RhoA-rescued mouse brain at P6 in which mitral cells were labed by tdTomato
Description
Z-series images of RhoA, RhoB, and RhoC knockout mouse brain at P6 in which mitral cells were labed by tdTomato. RhoA, B, and C were knocked out by using CRISPR-Cas9 and in utero electroporation. In this experoment, CRISPR- resistant-pCAG-RhoA was also introduced by in utero electroporation to test effect of the compensatory exprssion of RhoA.
Release, Updated
2026-08-07
License
CC BY 4.0
Kind
Image data
File Formats
.lif
Data size
1.8 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
neuron development neuron remodeling
Cellular Component (CC)
cell body dendrite
Biological Imaging Method
confocal microscopy
(
Fbbi:00000251
)
X scale
0.568 micrometer
Y scale
0.569 micrometer
Z scale
5 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T. Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Dev Cell. 2023 Jul 24;58(14):1221-1236.e7.
Related paper(s)
Contact
Takeshi Imai,
, Kyushu University,
, Graduate School of Medical Sciences,
, Graduate School of Medical Sciences,
Contributors
OMERO Project
Source