Detail of Fig3A_5dasLeafPrimordia_an3_04
Project
SSBD:Repository
Title
Z-series images of 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana_sample4 stained with BCECF-AM (vacuole) and FM4-64 (plasma membrane)
Description
The 5 DAS leaf primordia of an3 mutant-Arabidopsis thaliana sample4 stained with BCECF-AM, used as the fluorescent indicator for vacuole, and FM4-64, used as the dye for plasma membrane. Z-series images were obtained by confocal microscopy. The size and shape of vacuole in the mutant an3 were larger and more unified. The ANGUSTIFOLIA (AN3) gene is a positive regulator of cell proliferation and an3-4 allele was isolated by Horiguchi et al. (2005).
Release, Updated
2026-07-07
License
CC BY 4.0
Kind
Image data
File Formats
oir
Data size
231.5 MB
Organism
Arabidopsis thaliana
Strain(s)
an3-4
Cell Line
plasma membrane
Datatype
-
Molecular Function (MF)
Biological Process (BP)
cell population proliferation cell differentiation cell division
Cellular Component (CC)
vacuole plasma membrane
Biological Imaging Method
confocal microscopy (FBbi_00000251)
X scale
0.124 micrometer
Y scale
0.124 micrometer
Z scale
0.42 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Ezaki K, Koga H, Takeda-Kamiya N, Toyooka K, Higaki T, Sakamoto S, Tsukaya H. Precocious cell differentiation occurs in proliferating cells in leaf primordia in Arabidopsis angustifolia3 mutant. Front Plant Sci. 2024 Apr 16;15:1322223.
Related paper(s)
Contact
Kazune Ezaki, Hirokazu Tsukaya
, The University of Tokyo, The University of Tokyo
, Department of Biological Sciences, Graduate School of Science, Department of Biological Sciences, Graduate School of Science
, Department of Biological Sciences, Graduate School of Science, Department of Biological Sciences, Graduate School of Science
Contributors
OMERO Project
Source