Detail of Fig2_Rbp4_Ai162_MD_F6_210330_AP2
Project
SSBD:Repository
Title
Time-lapse calcium imaging showing the neural activity in OFC of female mouse at alloparental day 2
Description
The authors investigated the neural activity of the orbitofrontal cortex (OFC) during the parental behavior of retrieving pups at three maternal stages: AP1 (alloparental day 1), AP2 (alloparental day 1), and LM (lactating mother stage). A virgin female showing more than five pup-retrievals per session for the first time was defined as AP1 and the day following AP1 was defined as AP2. At AP1 and AP2, the pup-retrievals were examined using pups born from another female mouse. For the calcium imaging, the transgenic female mouse (Rbp4-Cre; Ai162 mouse) expressing GCaMP6s in the layer 5 pyramidal cells of the orbitofrontal cortex was used. The signal of GCaMP6s was obtained through gradient refractive index (GRIN) lens (500 micrometres in diameter) implanted above layer 5 of the OFC. This dataset includes the imaging data at AP2. The data at AP1 and LM obtained from the same female mouse are deposited in other datasets in this project; Fig2_Rbp4_Ai162_MD_F6_210329_AP1, and Fig2_Rbp4_Ai162_MD_F6_210421_LM.
Release, Updated
2026-06-26
License
CC BY 4.0
Kind
Image data
File Formats
tiff
Data size
22.8 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy (FBbi_00000246) Microendoscopic calcium imaging
X scale
0.625 micrometer
Y scale
0.602 micrometer
Z scale
-
T scale
0.1 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Tasaka GI, Hagihara M, Irie S, Kobayashi H, Inada K, Kobayashi K, Kato S, Kobayashi K, Miyamichi K. Orbitofrontal cortex influences dopamine dynamics associated with alloparental behavioral acquisition in female mice. Sci Adv. 2025 Jul 4;11(27):eadr4620.
Related paper(s)
Contact
Kazunari Miyamichi
, RIKEN BDR
Contributors
OMERO Project
Source