Detail of Fig2_Rbp4_Ai162_Gi_SMTb_F4_211013_AP2
Project
SSBD:Repository
Title
Time-lapse calcium imaging showing the neural activity in OFC of female mouse at alloparental day 2
Description
The authors investigated the neural activity of the orbitofrontal cortex (OFC) during the parental behavior of retrieving pups at three maternal stages: AP1 (alloparental day 1), AP2 (alloparental day 1), and LM (lactating mother stage). A virgin female showing more than five pup-retrievals per session for the first time was defined as AP1 and the day following AP1 was defined as AP2. At AP1 and AP2, the pup-retrievals were examined using pups born from another female mouse. For the calcium imaging, the transgenic female mouse (Rbp4-Cre; Ai162 mouse) expressing GCaMP6s in the layer 5 pyramidal cells of the orbitofrontal cortex was used. The signal of GCaMP6s was obtained through gradient refractive index (GRIN) lens (500 micrometres in diameter) implanted above layer 5 of the OFC. This dataset includes the imaging data at AP2. The data at AP1 and LM obtained from the same female mouse are deposited in other datasets in this project; Fig2_Rbp4_Ai162_Gi_SMTb_F4_211012_AP1, and Fig2_Rbp4_Ai162_Gi_SMTb_F4_211112_LM.
Release, Updated
2026-06-26
License
CC BY 4.0
Kind
Image data
File Formats
tiff
Data size
15.2 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
Biological Imaging Method
fluorescence microscopy (FBbi_00000246) Microendoscopic calcium imaging
X scale
0.625 micrometer
Y scale
0.602 micrometer
Z scale
-
T scale
0.1 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
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Objective model
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Filter set
-
Summary of Methods
Tasaka GI, Hagihara M, Irie S, Kobayashi H, Inada K, Kobayashi K, Kato S, Kobayashi K, Miyamichi K. Orbitofrontal cortex influences dopamine dynamics associated with alloparental behavioral acquisition in female mice. Sci Adv. 2025 Jul 4;11(27):eadr4620.
Related paper(s)
Gen-Ichi Tasaka, Mitsue Hagihara, Satsuki Irie, Haruna Kobayashi, Kengo Inada, Kenta Kobayashi, Shigeki Kato, Kazuto Kobayashi, Kazunari Miyamichi (2025) Orbitofrontal cortex influences dopamine dynamics associated with alloparental behavioral acquisition in female mice., Science advances, Volume 11, Number 27, pp. eadr4620
Published in July 2, 2025 (Electronic publication in July 2, 2025, midnight )
- doi: 10.1126/sciadv.adr4620
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pmid: 40601734
(Abstract) Maternal behaviors, which are crucial for the survival of mammalian infants, require the coordinated operation of multiple brain regions to process infant cues, make decisions, and execute motor plans. Although these processes likely demand higher cognitive functions, the prefrontal areas that regulate limbic parental programs remains poorly understood. Here, we show that the orbitofrontal cortex (OFC) excitatory projection neurons promote alloparental caregiving behaviors in female mice. By chronic microendoscopy, we observed robust yet adaptable representations of pup-directed anticipatory and motor-related activities within the OFC. Some of these plastic responses were significantly overlapped with those related to nonsocial reward signals. The inactivation of OFC output reduced the phasic activities of midbrain dopamine (DA) neurons specifically tied to pup retrieval and impaired the modulation of DA release to the ventral striatum during the acquisition of alloparental behaviors. These findings suggest that the OFC transiently boosts DA activity during the acquisition phase, thereby facilitating the manifestation of alloparental behaviors.(MeSH Terms)
Contact
Kazunari Miyamichi
, RIKEN BDR
Contributors
OMERO Project
Source