Detail of CaImage_TwichGmRR
Project
SSBD:Repository
Title
Calcium imaging of HeLa cells in response to histamine conducted with calcium indicator, Twitch-GmRR
Description
Calcium imaging of HeLa cells in response to histamine, which was conducted with calcium indicator, Twitch-GmRR. Twitch-GmRR Is an improved FRET-based calcium indicator with green and red fluorescent protein pairs.
Release, Updated
2026-06-09
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
305.3 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
response to histamine calcium ion import into cytosol
Cellular Component (CC)
cytosol
Biological Imaging Method
spinning disk confocal microscopy
(
Fbbi:00000253
)
X scale
1.875 micrometer
Y scale
1.875 micrometer
Z scale
-
T scale
3 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Matsuda T, Sakai S, Okazaki KI, Nagai T. Improvement of the Green-Red Forster Resonance Energy Transfer-Based Ca2+ Indicator by Using the Green Fluorescent Protein, Gamillus, with a Trans Chromophore as the Donor. ACS Sens . 2024 Apr 26;9(4):1743-1748.
Related paper(s)
Contact
Tomoki Matsuda, Takeharu Nagai
, Kitasato University, Osaka University
, School of Science, SANKEN
, School of Science, SANKEN
Contributors
OMERO Project