Detail of Fig3A_PIP2_before_EGF
Project
SSBD:Repository
Title
The tracjectories of JF549–PI(4,5)P2 from colocalization of EGFR and PIP2 in living cells before EGF stimulation
Description
Time-lapse images of Halo-PI(4,5)P_{2} ( Halo-PLCdelta-PH) on HeLa cells before EGF (epidermal growth factor) stimulation. To simultaneously observe the dynamics of both EGFR(epidermal growth factor receptor) molecules and PI(4,5)P_{2} (phosphatidylinositol-4,5-bisphosphate) molecules upon EGF stimulation, EGFR-GFP and Halo-PI(4,5)P_{2} were imaged using dual-color total internal reflection (TIRF) microscopy. Halo-PI(4,5)P_{2} was visualized with JF549, a HaloTag ligand. This dataset includes only Halo-PI(4,5)P_{2} images. The images of Halo-PI(4,5)P_{2} detected by JF549 fluorescence were deposited in another dataset in this project; Fig3A_EGFR_before_EGF
Release, Updated
2026-06-09
License
CC BY 4.0
Kind
Image data
File Formats
tif
Data size
100.0 MB
Datatype
-
Molecular Function (MF)
phosphatidylinositol-4
,
5-bisphosphate binding
Biological Process (BP)
regulation of epidermal growth factor receptor signaling pathway
Cellular Component (CC)
plasma membrane
Biological Imaging Method
evanescent wave microscopy
(
Fbbi:00000617
)
X scale
33.28 micrometer
Y scale
33.28 micrometer
Z scale
-
T scale
30 milliseconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Abe M, Yanagawa M, Hiroshima M, Kobayashi T, Sako Y. Bilateral regulation of EGFR activity and local PI(4,5)P_{2} dynamics in mammalian cells observed with superresolution microscopy. Elife. 2024 Nov 8;13:e101652.
Related paper(s)
Contact
Yasushi Sako
, RIKEN
, Cluster for Pioneering Research
, Cluster for Pioneering Research
Contributors
OMERO Project