Detail of Fig3A_EGFR_after_EGF
Project
SSBD:Repository
Title
The tracjectories of EGFR-GFP from colocalization of EGFR and PI(4,5)P2 in living cells after EGF stimulation
Description
Time-lapse images of EGFR-GFP (EGF receptor tagged with GFP) on HeLa cells between 1 and 5 min after EGF (epidermal growth factor) stimulation. To simultaneously observe the dynamics of both EGFR molecules and PI(4,5)P_{2} (phosphatidylinositol-4,5-bisphosphate) molecules upon EGF stimulation, EGFR-GFP and Halo-PI(4,5)P_{2} ( Halo-PLCdelta-PH) were imaged using dual-color total internal reflection (TIRF) microscopy. Halo-PI(4,5)P_{2} was visualized with JF549, a HaloTag ligand. This dataset includes only EGFR-GFP images. The images of Halo-PI(4,5)P_{2} detected by JF549 fluorescence were deposited in another dataset in this project; Fig3A_PIP2_after_EGF.
Release, Updated
2026-06-09
License
CC BY 4.0
Kind
Image data
File Formats
tif
Data size
100.0 MB
Datatype
-
Molecular Function (MF)
epidermal growth factor receptor activity
Biological Process (BP)
response to epidermal growth factor regulation of epidermal growth factor receptor signaling pathway
Cellular Component (CC)
plasma membrane
Biological Imaging Method
evanescent wave microscopy
(
Fbbi:00000617
)
X scale
33.28 micrometer
Y scale
33.28 micrometer
Z scale
-
T scale
30 milliseconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Abe M, Yanagawa M, Hiroshima M, Kobayashi T, Sako Y. Bilateral regulation of EGFR activity and local PI(4,5)P_{2} dynamics in mammalian cells observed with superresolution microscopy. Elife. 2024 Nov 8;13:e101652.
Related paper(s)
Contact
Yasushi Sako
, RIKEN
, Cluster for Pioneering Research
, Cluster for Pioneering Research
Contributors
OMERO Project