Detail of FigS1_HeLa_inhibit
Project
SSBD:Repository
Title
Time-lapse FRET images of Booster-PKA in reporter COX-DKO-HeLa cells expressing EP2 cocultured with producer HeLa cells in the presence of EP2/EP4 inhibitor
Description
Time-lapse FRET images of Booster-PKA in reporter COX-DKO (COX-1 and COX-2 deficient)-HeLa cells cocultured with producer HeLa cells. In the producer cells, DREADD-Gq and YC3.60 are expressed. After stimulation with DREADD-Gq ligand, PGE2 (prostaglandin E2) is generated in the producer cells. It is released and binds to the EP2 on the reporter cells and induces the FRET of PKA biosensor, Booster-PKA. These serial processes reveal the coverage of PGE2 around one activated cell. In this experiment, EP2/EP4 inhibitor was treated. In this dataset, the data of YC3.60 FRET is also reposited.
Release, Updated
2026-01-21
License
CC BY 4.0
Kind
Image data
File Formats
.nd2
Data size
213.1 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
cell communication
,
signal transduction
Cellular Component (CC)
cytoplasm
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
time lapse microscopy ( Fbbi:00000249 )
FRET ( Fbbi:00000367 )
time lapse microscopy ( Fbbi:00000249 )
FRET ( Fbbi:00000367 )
X scale
2.7 micrometer
Y scale
2.7 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Watabe T, Yamahira S, Matsuda M, Terai K. Visual quantification of prostaglandin E2 discharge from a single cell. Cell Struct Funct . 2023 Dec 7;48(2):241-249.
Related paper(s)
Contact
Kenta Terai
, Kyoto University
, Graduate School of Biostudies
, Laboratory of Bioimaging and Cell Signaling
Contributors
Tetsuya Watabe, Shinya Yamahira
OMERO Project