Detail of Fig3AB_PKA_MEKi_7
Project
SSBD:Repository
Title
Time-lapse FRET images of reporter HeLa cells and producer HeLa cells treated with MEK inhibitor
Description
Time-lapse FRET images of Booster-PKA in reporter COX-DKO (COX-1 and COX-2 deficient)-HeLa cells cocultured with producer HeLa cells. In the producer cells, optogenetic calcium channel stimulator: OptoSTIM1 (CRY2clust) is expressed, and PGE2 (prostaglandin E2) is generated after blue light flash. It is released and binds to the EP2 on the reporter cells and induces the FRET of PKA biosensor, Booster-PKA. These serial processes reveal the coverage of PGE2 around one activated cell. This dataset includs 5 files of serial imaging. The images of iRFP were obtained at the start and the end of the imaging showing the producer cells. 1 uM PD0325901 was applied 1 hour before imaging.
Release, Updated
2026-01-21
License
CC BY 4.0
Kind
Image data
File Formats
.nd2
Data size
553.6 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
cell communication
,
signal transduction
Cellular Component (CC)
cytoplasm
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
time lapse microscopy ( Fbbi:00000249 )
FRET ( Fbbi:00000367 )
time lapse microscopy ( Fbbi:00000249 )
FRET ( Fbbi:00000367 )
X scale
2.7 micrometer
Y scale
2.7 micrometer
Z scale
-
T scale
1 minute
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Watabe T, Yamahira S, Matsuda M, Terai K. Visual quantification of prostaglandin E2 discharge from a single cell. Cell Struct Funct . 2023 Dec 7;48(2):241-249.
Related paper(s)
Contact
Kenta Terai
, Kyoto University
, Graduate School of Biostudies
, Laboratory of Bioimaging and Cell Signaling
Contributors
Tetsuya Watabe, Shinya Yamahira
OMERO Project