SSBD:database

Detail of Fig3B_hdg11_12pdf2

Project

SSBD:Repository

Title

The time-lapse images of observing in vitro-cultivated hdg11/12 pdf2 ovules expressing WOX2p::H2B-GFP and WOX2p::LTI-tdTomato

Description

The marker WOX2p::H2B-GFP to label the embryo nucleus of Arabidopsis and the marker WOX2p::LTI-tdTomato to label the plasma membrane (PM) showed that after the zygote division the apical cell of in vitro-cultivated hdg11/12 pdf2 ovules expands to take on a special shape before dividing vertically. The pdf2 mutant was introduced into the hdg11/12 mutant to get the hdg11/12 pdf2 triple mutant.

Release, Updated

2026-01-21

License

CC BY 4.0

Kind

Image data

File Formats

.tif

Data size

2.8 GB

Organism

Arabidopsis thaliana

Strain(s)

Col-0

Cell Line

nucleus plasma membrane

Datatype

Molecular Function (MF)

Biological Process (BP)

zygote elongation

Cellular Component (CC)

nucleus plasma membrane

Biological Imaging Method

spinning disk confocal microscopy (FBbi_00000253)

X scale

2.0 micrometer

Y scale

2.0 micrometer

Z scale

5.0 micrometer

T scale

10 minutes

Image Acquisition

Experiment type

Microscope type

Acquisition mode

Contrast method

Microscope model

Detector model

Objective model

Filter set

Summary of Methods

Tanaka S, Matsushita Y, Hanaki Y, Higaki T, Kamamoto N, Matsushita K, Higashiyama T, Fujimoto K, Ueda M. HD-ZIP IV genes are essential for embryo initial cell polarization and the radial axis formation in Arabidopsis. Curr Biol. 2024 Oct 21;34(20):4639-4649.e4.